Key features and details
- Rabbit polyclonal to PHF11
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PHF11 antibody
See all PHF11 primary antibodies
DescriptionRabbit polyclonal to PHF11
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human PHF11 aa 1-292.
Database link: Q9UIL8
- WB: Jurkat, HEK-293T and HeLa whole cell lysate. IHC-P: Human testis and placenta tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230233 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 38, 33 kDa).|
FunctionPositive regulator of Th1-type cytokine gene expression.
Tissue specificityHighly expressed in T and B-cells, as well as natural killer and mature dendritic cells. Expressed at higher levels in Th1 as compared to Th2 cells. Expressed at low levels in all normal tissues tested, including lung, testis, small intestine, breast, liver and placenta.
Sequence similaritiesContains 1 PHD-type zinc finger.
- Information by UniProt
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All lanes : Anti-PHF11 antibody (ab230233) at 1/1000 dilution
Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 38, 33 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Paraffin-embedded human testis tissue stained for PHF11 with ab230233 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human placenta tissue stained for PHF11 with ab230233 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230233 has not yet been referenced specifically in any publications.