Key features and details
- Rabbit polyclonal to PHF16
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PHF16 antibody
DescriptionRabbit polyclonal to PHF16
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 773-823 of Human PHF16 (NP_055550.1).
- HeLa, 293T and Jurkat whole cell lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab129495 was affinity purified using an epitope specific to PHF16 immobilized on solid support.
Our Abpromise guarantee covers the use of ab129495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 94 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionComponent of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo.
Tissue specificityUbiquitously expressed, with highest levels in placenta and uterus.
Sequence similaritiesBelongs to the JADE family.
Contains 1 PHD-type zinc finger.
- Information by UniProt
- JADE 3 antibody
- Jade family PHD finger protein 3 antibody
- JADE3 antibody
All lanes : Anti-PHF16 antibody (ab129495) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 94 kDa
Exposure time: 3 minutes
Detection of PHF16 by Western Blot of Immunprecipitate.
ab129495 at 1 µg/ml staining PHF16 in HeLa whole cell lysate immunoprecipitated using ab129495 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: control IgG.
Predicted band size : 94 kDa.
Detection: Chemiluminescence with exposure time of 3 minutes.
ab129495 has been referenced in 1 publication.
- Pardo M et al. Myst2/Kat7 histone acetyltransferase interaction proteomics reveals tumour-suppressor Niam as a novel binding partner in embryonic stem cells. Sci Rep 7:8157 (2017). PubMed: 28811661