Overview

  • Product name

    Anti-PHF16 antibody
  • Description

    Rabbit polyclonal to PHF16
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 773-823 of Human PHF16 (NP_055550.1).

  • Positive control

    • HeLa, 293T and Jurkat whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7-8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab129495 was affinity purified using an epitope specific to PHF16 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab129495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 94 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo.
  • Tissue specificity

    Ubiquitously expressed, with highest levels in placenta and uterus.
  • Sequence similarities

    Belongs to the JADE family.
    Contains 1 PHD-type zinc finger.
  • Information by UniProt
  • Database links

  • Alternative names

    • JADE 3 antibody
    • Jade family PHD finger protein 3 antibody
    • JADE3 antibody
    • JADE3_HUMAN antibody
    • KIAA0215 antibody
    • MGC138748 antibody
    • MGC138749 antibody
    • PHD finger protein 16 antibody
    • PHF 16 antibody
    • Phf16 antibody
    • Protein Jade 3 antibody
    • Protein Jade-3 antibody
    see all

Images

  • All lanes : Anti-PHF16 antibody (ab129495) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 94 kDa


    Exposure time: 3 minutes
  • Detection of PHF16 by Western Blot of Immunprecipitate.
    ab129495 at 1 µg/ml staining PHF16 in HeLa whole cell lysate immunoprecipitated using ab129495 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Lane 2: control IgG.
    Predicted band size : 94 kDa.
    Detection: Chemiluminescence with exposure time of 3 minutes.

References

This product has been referenced in:

  • Pardo M  et al. Myst2/Kat7 histone acetyltransferase interaction proteomics reveals tumour-suppressor Niam as a novel binding partner in embryonic stem cells. Sci Rep 7:8157 (2017). Read more (PubMed: 28811661) »
See 1 Publication for this product

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There are currently no Customer reviews or Questions for ab129495.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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