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    phf17-antibody-n-terminal-ab155215.pdf

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Epigenetics and Nuclear Signaling Transcription Other factors
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Anti-PHF17 antibody - N-terminal (ab155215)

  • Datasheet
  • SDS
Submit a review Submit a question References (1)

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Western blot - Anti-PHF17 antibody - N-terminal (ab155215)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF17 antibody - N-terminal (ab155215)
  • Immunocytochemistry/ Immunofluorescence - Anti-PHF17 antibody - N-terminal (ab155215)

Key features and details

  • Rabbit polyclonal to PHF17 - N-terminal
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-PHF17 antibody - N-terminal
    See all PHF17 primary antibodies
  • Description

    Rabbit polyclonal to PHF17 - N-terminal
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment, corresponding to a region within N terminal amino acids 5-241 of Human PHF17 (UniProt: Q6IE81).

  • Positive control

    • A549, HeLa and HepG2 whole cell lysates; Human MDA-MB157 xenograft tissue; HeLa cells.
  • General notes

    Store as concentrated solution.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HAT

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • A549 whole cell lysate (ab7910)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Human
All applications
Mouse
Rat
Cow
Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 96 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 96 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Transcriptional coactivator it may also promote acetylation of nucleosomal histone H4 by KAT5. Promotes apoptosis. May act as a renal tumor suppressor.
  • Tissue specificity

    Highly expressed in kidney. Also present in pancreas, liver and heart (at protein level). Down-regulated in renal cancer cells.
  • Sequence similarities

    Belongs to the JADE family.
    Contains 2 PHD-type zinc fingers.
  • Domain

    The 2 PHD-type zinc fingers are required for transcriptional activity.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Target information above from: UniProt accession Q6IE81 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 509264 Cow
    • Entrez Gene: 79960 Human
    • Entrez Gene: 269424 Mouse
    • Entrez Gene: 310352 Rat
    • Omim: 610514 Human
    • SwissProt: Q5E9T7 Cow
    • SwissProt: Q6IE81 Human
    • SwissProt: Q6ZPI0 Mouse
    • Unigene: 12420 Human
    • Unigene: 286285 Mouse
    • Unigene: 90858 Rat
    see all
  • Alternative names

    • FLJ22479 antibody
    • Gene for apoptosis and differentiation in epithelia antibody
    • Jade family PHD finger 1 antibody
    • JADE1 antibody
    • JADE1_HUMAN antibody
    • KIAA1807 antibody
    • OTTHUMP00000219842 antibody
    • OTTHUMP00000219934 antibody
    • OTTHUMP00000219935 antibody
    • OTTHUMP00000219936 antibody
    • PHD finger protein 17 antibody
    • PHD protein Jade 1 antibody
    • Phf17 antibody
    • Protein Jade 1 antibody
    • Protein Jade-1 antibody
    see all

Images

  • Western blot - Anti-PHF17 antibody - N-terminal (ab155215)
    Western blot - Anti-PHF17 antibody - N-terminal (ab155215)
    Anti-PHF17 antibody - N-terminal (ab155215) at 1/5000 dilution + A549 whole cell lysate at 30 µg

    Predicted band size: 96 kDa



    7.5% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF17 antibody - N-terminal (ab155215)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF17 antibody - N-terminal (ab155215)
    Immunohistochemical analysis of paraffin-embedded Human MDA-MB157 xenograft tissue labeling PHF17 with ab155215 at 1/500 dilution.
  • Immunocytochemistry/ Immunofluorescence - Anti-PHF17 antibody - N-terminal (ab155215)
    Immunocytochemistry/ Immunofluorescence - Anti-PHF17 antibody - N-terminal (ab155215)
    Confocal immunofluorescent analysis of Paraformaldehyde-fixed HeLa cells labeling PHF17 with ab155215 at 1/500 dilution (green). Alpha-tubulin filaments are labeled red.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab155215? Please let us know so that we can cite the reference in this datasheet.

    ab155215 has been referenced in 1 publication.

    • Zhou J  et al. Silencing of microRNA-135b inhibits invasion, migration, and stemness of CD24+CD44+ pancreatic cancer stem cells through JADE-1-dependent AKT/mTOR pathway. Cancer Cell Int 20:134 (2020). PubMed: 32351328

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