Anti-PHF17 antibody - N-terminal (ab155215)
Key features and details
- Rabbit polyclonal to PHF17 - N-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PHF17 antibody - N-terminal
See all PHF17 primary antibodies -
Description
Rabbit polyclonal to PHF17 - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Recombinant fragment, corresponding to a region within N terminal amino acids 5-241 of Human PHF17 (UniProt: Q6IE81).
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Positive control
- A549, HeLa and HepG2 whole cell lysates; Human MDA-MB157 xenograft tissue; HeLa cells.
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General notes
Store as concentrated solution.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155215 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| All applications |
Mouse
Rat
Cow
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 96 kDa.
|
|
| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| ICC/IF |
1/100 - 1/1000.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 96 kDa. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
1/100 - 1/1000. |
Target
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Function
Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Transcriptional coactivator it may also promote acetylation of nucleosomal histone H4 by KAT5. Promotes apoptosis. May act as a renal tumor suppressor. -
Tissue specificity
Highly expressed in kidney. Also present in pancreas, liver and heart (at protein level). Down-regulated in renal cancer cells. -
Sequence similarities
Belongs to the JADE family.
Contains 2 PHD-type zinc fingers. -
Domain
The 2 PHD-type zinc fingers are required for transcriptional activity. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 509264 Cow
- Entrez Gene: 79960 Human
- Entrez Gene: 269424 Mouse
- Entrez Gene: 310352 Rat
- Omim: 610514 Human
- SwissProt: Q5E9T7 Cow
- SwissProt: Q6IE81 Human
- SwissProt: Q6ZPI0 Mouse
see all -
Alternative names
- FLJ22479 antibody
- Gene for apoptosis and differentiation in epithelia antibody
- Jade family PHD finger 1 antibody
see all
Images
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Western blot - Anti-PHF17 antibody - N-terminal (ab155215)Anti-PHF17 antibody - N-terminal (ab155215) at 1/5000 dilution + A549 whole cell lysate at 30 µg
Predicted band size: 96 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF17 antibody - N-terminal (ab155215)Immunohistochemical analysis of paraffin-embedded Human MDA-MB157 xenograft tissue labeling PHF17 with ab155215 at 1/500 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-PHF17 antibody - N-terminal (ab155215)Confocal immunofluorescent analysis of Paraformaldehyde-fixed HeLa cells labeling PHF17 with ab155215 at 1/500 dilution (green). Alpha-tubulin filaments are labeled red.
Protocols
References (1)
ab155215 has been referenced in 1 publication.
- Zhou J et al. Silencing of microRNA-135b inhibits invasion, migration, and stemness of CD24+CD44+ pancreatic cancer stem cells through JADE-1-dependent AKT/mTOR pathway. Cancer Cell Int 20:134 (2020). PubMed: 32351328
