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Rabbit polyclonal to PHF17 - N-terminal
Predicted to work with:
Mouse, Rat, Cow
Recombinant fragment, corresponding to a region within N terminal amino acids 5-241 of Human PHF17 (UniProt: Q6IE81).
A549, HeLa and HepG2 whole cell lysates; Human MDA-MB157 xenograft tissue; HeLa cells.
Store as concentrated solution.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 78% PBS, 20% Glycerol, 1% BSA
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 96 kDa.
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/100 - 1/1000.
Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Transcriptional coactivator it may also promote acetylation of nucleosomal histone H4 by KAT5. Promotes apoptosis. May act as a renal tumor suppressor.
Highly expressed in kidney. Also present in pancreas, liver and heart (at protein level). Down-regulated in renal cancer cells.
Belongs to the JADE family.
Contains 2 PHD-type zinc fingers.
The 2 PHD-type zinc fingers are required for transcriptional activity.
Information by UniProt
Gene for apoptosis and differentiation in epithelia antibody
Jade family PHD finger 1 antibody
Western blot - Anti-PHF17 antibody - N-terminal (ab155215)
Anti-PHF17 antibody - N-terminal (ab155215) at 1/5000 dilution + A549 whole cell lysate at 30 µg
Predicted band size: 96 kDa 7.5% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF17 antibody - N-terminal (ab155215)
Immunohistochemical analysis of paraffin-embedded Human MDA-MB157 xenograft tissue labeling PHF17 with ab155215 at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-PHF17 antibody - N-terminal (ab155215)
Confocal immunofluorescent analysis of Paraformaldehyde-fixed HeLa cells labeling PHF17 with ab155215 at 1/500 dilution (green). Alpha-tubulin filaments are labeled red.
has not yet been referenced specifically in any publications.
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