Overview

  • Product name
    Anti-PHF17 antibody - N-terminal
    See all PHF17 primary antibodies
  • Description
    Rabbit polyclonal to PHF17 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within N terminal amino acids 5-241 of Human PHF17 (UniProt: Q6IE81).

  • Positive control
    • A549, HeLa and HepG2 whole cell lysates; Human MDA-MB157 xenograft tissue; HeLa cells.
  • General notes
    Store as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab155215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 96 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Transcriptional coactivator it may also promote acetylation of nucleosomal histone H4 by KAT5. Promotes apoptosis. May act as a renal tumor suppressor.
  • Tissue specificity
    Highly expressed in kidney. Also present in pancreas, liver and heart (at protein level). Down-regulated in renal cancer cells.
  • Sequence similarities
    Belongs to the JADE family.
    Contains 2 PHD-type zinc fingers.
  • Domain
    The 2 PHD-type zinc fingers are required for transcriptional activity.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • FLJ22479 antibody
    • Gene for apoptosis and differentiation in epithelia antibody
    • Jade family PHD finger 1 antibody
    • JADE1 antibody
    • JADE1_HUMAN antibody
    • KIAA1807 antibody
    • OTTHUMP00000219842 antibody
    • OTTHUMP00000219934 antibody
    • OTTHUMP00000219935 antibody
    • OTTHUMP00000219936 antibody
    • PHD finger protein 17 antibody
    • PHD protein Jade 1 antibody
    • Phf17 antibody
    • Protein Jade 1 antibody
    • Protein Jade-1 antibody
    see all

Images

  • Western blot - Anti-PHF17 antibody - N-terminal (ab155215)
    Western blot - Anti-PHF17 antibody - N-terminal (ab155215)
    Anti-PHF17 antibody - N-terminal (ab155215) at 1/5000 dilution + A549 whole cell lysate at 30 µg

    Predicted band size: 96 kDa



    7.5% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF17 antibody - N-terminal (ab155215)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF17 antibody - N-terminal (ab155215)
    Immunohistochemical analysis of paraffin-embedded Human MDA-MB157 xenograft tissue labeling PHF17 with ab155215 at 1/500 dilution.
  • Immunocytochemistry/ Immunofluorescence - Anti-PHF17 antibody - N-terminal (ab155215)
    Immunocytochemistry/ Immunofluorescence - Anti-PHF17 antibody - N-terminal (ab155215)
    Confocal immunofluorescent analysis of Paraformaldehyde-fixed HeLa cells labeling PHF17 with ab155215 at 1/500 dilution (green). Alpha-tubulin filaments are labeled red.

References

ab155215 has not yet been referenced specifically in any publications.

Publishing research using ab155215? Please let us know so that we can cite the reference in this datasheet.

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