1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Possible transcription factor.
Expressed in heart, kidney, liver, lung, pancreas, placenta, spleen and testis. Not expressed in brain, skeletal muscle, colon, ovary, prostate, small intestine and thymus. Expressed in colon and ovary cancer cell lines while it is not expressed in the respective normal tissues.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue labelling PHF20 with ab170825 at 1/50 dilution, followed by peroxidase-conjugated secondary antibody and DAB staining.