Key features and details
- Mouse polyclonal to PHF20L1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PHF20L1 antibody
See all PHF20L1 primary antibodies
DescriptionMouse polyclonal to PHF20L1
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Full length PHF20L1 protein (Human)
- PHF20L1 transfected 293T cell lysate and Hela cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.4
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab67796 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 46 kDa).|
|ICC/IF||Use a concentration of 10 µg/ml.|
Sequence similaritiesContains 1 PHD-type zinc finger.
Contains 2 Tudor domains.
- Information by UniProt
- CGI 72 antibody
- MGC64923 antibody
- OTTHUMP00000192870 antibody
All lanes : Anti-PHF20L1 antibody (ab67796) at 1/500 dilution
Lane 1 : PHF20L1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 46 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
ab67796, at 10µg/ml, staining PHF20L1 in Hela cell
ab67796 has been referenced in 1 publication.
- Estève PO et al. Methyllysine reader plant homeodomain (PHD) finger protein 20-like 1 (PHF20L1) antagonizes DNA (cytosine-5) methyltransferase 1 (DNMT1) proteasomal degradation. J Biol Chem 289:8277-87 (2014). ICC/IF ; Human . PubMed: 24492612