Overview

  • Product name

    Anti-PHF21B antibody [OTI3D1]
  • Description

    Mouse monoclonal [OTI3D1] to PHF21B
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human PHF21B. Produced in HEK293T cells (NP_612424.1).
    Database link: Q96EK2

  • Positive control

    • IHC-P: Human Breast, Kidney, Endometrium, Liver, Ovary Adenocarcinoma, Pancreas carcinoma, Lung carcinoma and Thyroid Carcinoma tissues. WB: HEK-293T cell lysate transfected with pCMV6-ENTRY PHF21B cDNA. ICC/IF: COS7 cells transiently transfected by pCMV6-ENTRY PHF21B. Flow: HEK-293T cells transfected with pCMV6-ENTRY PHF21B overexpress plasmid.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    Purified from cell culture supernatant by affinity chromatography.
  • Clonality

    Monoclonal
  • Clone number

    OTI3D1
  • Isotype

    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab119249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 57 kDa.
IHC-P 1/150.
Flow Cyt 1/100.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100.

Target

  • Sequence similarities

    Contains 1 PHD-type zinc finger.
  • Information by UniProt
  • Database links

  • Alternative names

    • A730032D07Rik antibody
    • AL049760.6 antibody
    • BHC80L antibody
    • FLJ34161 antibody
    • OTTHUMP00000199355 antibody
    • OTTHUMP00000199357 antibody
    • PF21B_HUMAN antibody
    • PHD finger protein 21B antibody
    • PHD finger protein 4 antibody
    • Phf21b antibody
    • PHF4 antibody
    • RGD1308739 antibody
    see all

Images

  • All lanes : Anti-PHF21B antibody [OTI3D1] (ab119249) at 1/2000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY
    control cDNA
    Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY
    PHF21B cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 57 kDa

  • Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either pCMV6-ENTRY PHF21B overexpress plasmid (Red) or empty vector control plasmid (Blue), stained using ab119249 at 1/100 dilution.

  • COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY PHF21B, stained for PHF21B (green) using ab119249 at 1/100 dilution in ICC/IF.

     

  • Paraffin-embedded human breast tissue stained for PHF21B using ab119249 at 1/150 dilution in immunohistochemical analysis.

  • Paraffin-embedded human endometrium tissue stained for PHF21B using ab119249 at 1/150 dilution in immunohistochemical analysis.

  • Paraffin-embedded human kidney tissue stained for PHF21B using ab119249 at 1/150 dilution in immunohistochemical analysis.

  • Paraffin-embedded human liver tissue stained for PHF21B using ab119249 at 1/150 dilution in immunohistochemical analysis.

  • Paraffin-embedded human lung carcinoma tissue stained for PHF21B using ab119249 at 1/150 dilution in immunohistochemical analysis.

  • Paraffin-embedded human ovary adenocarcinoma tissue stained for PHF21B using ab119249 at 1/150 dilution in immunohistochemical analysis.

     

  • Paraffin-embedded human pancreas carcinoma tissue stained for PHF21B using ab119249 at 1/150 dilution in immunohistochemical analysis.

  • Paraffin-embedded human breast thyroid carcinoma tissue stained for PHF21B using ab119249 at 1/150 dilution in immunohistochemical analysis.

References

ab119249 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (ES cells)
Gel Running Conditions
Non-reduced Denaturing (10% gel)
Loading amount
60 µg
Treatment
0.5µg/ml Doxycyclin for 48h
Specification
ES cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted May 19 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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