Product nameAnti-PHF23 antibody
See all PHF23 primary antibodies
DescriptionRabbit polyclonal to PHF23
Tested applicationsSuitable for: IP, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Cow
Synthetic peptide within Human PHF23 aa 353-403. The exact sequence is proprietary. NP_077273.2
Database link: Q9BUL5
- IHC-P: Human prostate carcinoma. WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264292 in the following tested applications.
|IP||Use at 10 µg/mg of lysate.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|WB||1/2000 - 1/10000.|
Involvement in diseaseNote=A chromosomal aberration involving PHF23 is found in a patient with acute myeloid leukemia (AML). Translocation t(11;17)(p15;p13) with NUP98.
Sequence similaritiesBelongs to the PHF23 family.
Contains 1 PHD-type zinc finger.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- 410141M05Rik antibody
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All lanes : Anti-PHF23 antibody (ab264292) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Exposure time: 30 seconds
PHF23 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264292 at 10 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264292 at 0.4 µg/ml.
Lane 1: ab264292 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 30 secs.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling PHF23 with ab264292 at 1/1,000 dilution. Detection: DAB.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
ab264292 has not yet been referenced specifically in any publications.