Product nameAnti-PHF24 antibody
DescriptionRabbit polyclonal to PHF24
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
- RT4, U 251 MG, Human Liver and Human Tonsil lysates; Human pancreas tissue.
This product was previously labelled as KIAA1045
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121643 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/250 - 1/500.|
Sequence similaritiesContains 1 PHD-type zinc finger.
- Information by UniProt
- K1045_HUMAN antibody
- Kiaa1045 antibody
- Protein KIAA1045 antibody
- RP11-392A14.4 antibody
All lanes : Anti-PHF24 antibody (ab121643) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U 251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
10-20% SDS PAGE
Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and vesicles. Recommended concentration of ab121643 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
ab121643 at 1/500 dilution staining PHF24 in Paraffin-embedded Human pancreas tissue by Immunohistochemistry.
ab121643 has not yet been referenced specifically in any publications.