Overview

  • Product name

    Anti-PHGDH/Malate dehydrogenase antibody
    See all PHGDH/Malate dehydrogenase primary antibodies
  • Description

    Rabbit polyclonal to PHGDH/Malate dehydrogenase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human PHGDH/Malate dehydrogenase aa 209-397 (internal sequence).
    Database link: NP_006614

  • Positive control

    • 293T, Raji, A431 and Jurkat whole cell lysates; Human breast carcinoma tissue.
  • General notes

     This product was previously labelled as PHGDH

     

Properties

Applications

Our Abpromise guarantee covers the use of ab125865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 57 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Pathway

    Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3.
  • Involvement in disease

    Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency (PHGDH deficiency) [MIM:601815]. It is characterized by congenital microcephaly, psychomotor retardation, and seizures.
  • Sequence similarities

    Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3 PGDH antibody
    • 3-PGDH antibody
    • 3-phosphoglycerate dehydrogenase antibody
    • 3PGDH antibody
    • D-3-phosphoglycerate dehydrogenase antibody
    • EC 1.1.1.95 antibody
    • Epididymis secretory protein Li 113 antibody
    • HEL S 113 antibody
    • NLS antibody
    • NLS1 antibody
    • PDG antibody
    • PGAD antibody
    • PGD antibody
    • PGDH antibody
    • PGDH3 antibody
    • Phgdh antibody
    • PHGDHD antibody
    • Phosphoglycerate dehydrogenase antibody
    • SERA antibody
    • SERA_HUMAN antibody
    see all

Images

  • All lanes : Anti-PHGDH/Malate dehydrogenase antibody (ab125865) at 1/1000 dilution

    Lane 1 : WT HeLa cell extract
    Lane 2 : PHGDH/Malate dehydrogenase Knockout HeLa cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 57 kDa

  • All lanes : Anti-PHGDH/Malate dehydrogenase antibody (ab125865) at 1/10000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 57 kDa



    7.5% SDS PAGE
  • ab125865, at 1/250, staining PHGDH/Malate dehydrogenase in formalin fixed, paraffin embedded Human breast carcinoma tissue by Immunohistochemistry.

References

ab125865 has not yet been referenced specifically in any publications.

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