Overview

  • Product name

    Anti-PHGDH/Malate dehydrogenase antibody
    See all PHGDH/Malate dehydrogenase primary antibodies
  • Description

    Rabbit polyclonal to PHGDH/Malate dehydrogenase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Horse, Pig, Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human PHGDH/Malate dehydrogenase aa 500 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab156055)

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; A431; Raji; MCF7. IF/ICC: MCF7 cell line.
  • General notes

     This product was previously labelled as PHGDH

     

Properties

Applications

Our Abpromise guarantee covers the use of ab133133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).

Target

  • Pathway

    Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3.
  • Involvement in disease

    Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency (PHGDH deficiency) [MIM:601815]. It is characterized by congenital microcephaly, psychomotor retardation, and seizures.
  • Sequence similarities

    Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3 PGDH antibody
    • 3-PGDH antibody
    • 3-phosphoglycerate dehydrogenase antibody
    • 3PGDH antibody
    • D-3-phosphoglycerate dehydrogenase antibody
    • EC 1.1.1.95 antibody
    • Epididymis secretory protein Li 113 antibody
    • HEL S 113 antibody
    • NLS antibody
    • NLS1 antibody
    • PDG antibody
    • PGAD antibody
    • PGD antibody
    • PGDH antibody
    • PGDH3 antibody
    • Phgdh antibody
    • PHGDHD antibody
    • Phosphoglycerate dehydrogenase antibody
    • SERA antibody
    • SERA_HUMAN antibody
    see all

Images

  • ICC/IF image of ab133133 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab133133, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit  IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) Hek293 and MCF7 cells at 10µg/ml.

  • All lanes : Anti-PHGDH/Malate dehydrogenase antibody (ab133133) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 56 kDa


    Exposure time: 5 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab133133 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab133133 has not yet been referenced specifically in any publications.

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