• Product name

    Anti-PHGDH/Malate dehydrogenase antibody
    See all PHGDH/Malate dehydrogenase primary antibodies
  • Description

    Rabbit polyclonal to PHGDH/Malate dehydrogenase
  • Host species

  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Horse, Pig, Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human PHGDH/Malate dehydrogenase aa 500 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab156055)

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; A431; Raji; MCF7. IF/ICC: MCF7 cell line.
  • General notes

     This product was previously labelled as PHGDH




Our Abpromise guarantee covers the use of ab133133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).


  • Pathway

    Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3.
  • Involvement in disease

    Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency (PHGDH deficiency) [MIM:601815]. It is characterized by congenital microcephaly, psychomotor retardation, and seizures.
  • Sequence similarities

    Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3 PGDH antibody
    • 3-PGDH antibody
    • 3-phosphoglycerate dehydrogenase antibody
    • 3PGDH antibody
    • D-3-phosphoglycerate dehydrogenase antibody
    • EC antibody
    • Epididymis secretory protein Li 113 antibody
    • HEL S 113 antibody
    • NLS antibody
    • NLS1 antibody
    • PDG antibody
    • PGAD antibody
    • PGD antibody
    • PGDH antibody
    • PGDH3 antibody
    • Phgdh antibody
    • PHGDHD antibody
    • Phosphoglycerate dehydrogenase antibody
    • SERA antibody
    • SERA_HUMAN antibody
    see all


  • ICC/IF image of ab133133 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab133133, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit  IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) Hek293 and MCF7 cells at 10µg/ml.

  • All lanes : Anti-PHGDH/Malate dehydrogenase antibody (ab133133) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 56 kDa

    Exposure time: 5 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab133133 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.


ab133133 has not yet been referenced specifically in any publications.

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