Key features and details
- Rabbit polyclonal to PHGDH/Malate dehydrogenase
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PHGDH/Malate dehydrogenase antibody
See all PHGDH/Malate dehydrogenase primary antibodies
DescriptionRabbit polyclonal to PHGDH/Malate dehydrogenase
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow, Pig, Chimpanzee, Orangutan
Synthetic peptide within Human PHGDH/Malate dehydrogenase aa 1-50. The exact sequence is proprietary. (NP_006614.2).
Database link: O43175
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate. IP: PHGDH/Malate dehydrogenase in HEK-293T whole cell lysate. IHC-P: Human breast carcinoma and mouse teratoma tissue.
This product was previously labelled as PHGDH
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab240744 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000.|
|IP||Use at 2-10 µg/mg of lysate.|
PathwayAmino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3.
Involvement in diseaseDefects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency (PHGDH deficiency) [MIM:601815]. It is characterized by congenital microcephaly, psychomotor retardation, and seizures.
Sequence similaritiesBelongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
- Information by UniProt
- 3 PGDH antibody
- 3-PGDH antibody
- 3-phosphoglycerate dehydrogenase antibody
All lanes : Anti-PHGDH/Malate dehydrogenase antibody (ab240744) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Exposure time: 10 seconds
Lysates prepared using NETN lysis buffer.
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for PHGDH/Malate dehydrogenase using ab240744 at 1 µg/ml in immunohistochemical analysis. DAB staining.
PHGDH/Malate dehydrogenase was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab240744 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab240744 at 0.4 µg/ml.
Lane 1: ab240744 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
Formalin-fixed, paraffin-embedded mouse teratoma tissue stained for PHGDH/Malate dehydrogenase using ab240744 at 1 µg/ml in immunohistochemical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab240744 has not yet been referenced specifically in any publications.