Overview

  • Product name

    Anti-PHGDH/Malate dehydrogenase antibody
    See all PHGDH/Malate dehydrogenase primary antibodies
  • Description

    Rabbit polyclonal to PHGDH/Malate dehydrogenase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Cow, Pig, Chimpanzee, Orangutan
  • Immunogen

    Synthetic peptide within Human PHGDH/Malate dehydrogenase aa 1-50. The exact sequence is proprietary. (NP_006614.2).
    Database link: O43175

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate. IP: PHGDH/Malate dehydrogenase in HEK-293T whole cell lysate. IHC-P: Human breast carcinoma and mouse teratoma tissue.
  • General notes

     This product was previously labelled as PHGDH

     

Properties

Applications

Our Abpromise guarantee covers the use of ab240744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000.
IP Use at 2-10 µg/mg of lysate.

Target

Images

  • All lanes : Anti-PHGDH/Malate dehydrogenase antibody (ab240744) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Exposure time: 10 seconds


    Lysates prepared using NETN lysis buffer.

  • Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for PHGDH/Malate dehydrogenase using ab240744 at 1 µg/ml in immunohistochemical analysis. DAB staining.

  • PHGDH/Malate dehydrogenase was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab240744 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab240744 at 0.4 µg/ml.

    Lane 1: ab240744 IP in HEK-293T whole cell lysate.
    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with exposure time of 10 seconds.

  • Formalin-fixed, paraffin-embedded mouse teratoma tissue stained for PHGDH/Malate dehydrogenase using ab240744 at 1 µg/ml in immunohistochemical analysis. DAB staining.

References

ab240744 has not yet been referenced specifically in any publications.

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