Product nameAnti-PHGDH/Malate dehydrogenase antibody
See all PHGDH/Malate dehydrogenase primary antibodies
DescriptionRabbit polyclonal to PHGDH/Malate dehydrogenase
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow, Pig, Chimpanzee, Orangutan
Synthetic peptide within Human PHGDH/Malate dehydrogenase aa 1-50. The exact sequence is proprietary. (NP_006614.2).
Database link: O43175
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate. IP: PHGDH/Malate dehydrogenase in HEK-293T whole cell lysate. IHC-P: Human breast carcinoma and mouse teratoma tissue.
This product was previously labelled as PHGDH
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab240744 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000.|
|IP||Use at 2-10 µg/mg of lysate.|
PathwayAmino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3.
Involvement in diseaseDefects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency (PHGDH deficiency) [MIM:601815]. It is characterized by congenital microcephaly, psychomotor retardation, and seizures.
Sequence similaritiesBelongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
- Information by UniProt
- 3 PGDH antibody
- 3-PGDH antibody
- 3-phosphoglycerate dehydrogenase antibody
All lanes : Anti-PHGDH/Malate dehydrogenase antibody (ab240744) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Exposure time: 10 seconds
Lysates prepared using NETN lysis buffer.
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for PHGDH/Malate dehydrogenase using ab240744 at 1 µg/ml in immunohistochemical analysis. DAB staining.
PHGDH/Malate dehydrogenase was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab240744 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab240744 at 0.4 µg/ml.
Lane 1: ab240744 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
Formalin-fixed, paraffin-embedded mouse teratoma tissue stained for PHGDH/Malate dehydrogenase using ab240744 at 1 µg/ml in immunohistochemical analysis. DAB staining.
ab240744 has not yet been referenced specifically in any publications.