Overview

  • Product name

  • Description

    Rabbit polyclonal to PHKA1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Recombinant fragment within Human PHKA1 (internal sequence). The exact sequence is proprietary.
    Database link: P46020

  • Positive control

    • WB: HEK-293T whole cell extract. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab228593 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 137 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin.
  • Tissue specificity

    Muscle specific. Isoform 1 is predominant in vastus lateralis muscle. Isoform 2 predominates slightly in heart, and it predominates clearly in the other tissues tested.
  • Pathway

    Glycan biosynthesis; glycogen metabolism.
  • Involvement in disease

    Glycogen storage disease 9D (GSD9D) [MIM:300559]: A metabolic disorder characterized by slowly progressive, predominantly distal muscle weakness and atrophy. Clinical features include exercise intolerance with early fatigability, pain, cramps and occasionally myoglobinuria. Note=The disease is caused by mutations affecting the gene represented in this entry.
  • Sequence similarities

    Belongs to the phosphorylase b kinase regulatory chain family.
  • Post-translational
    modifications

    Although the final Cys may be farnesylated, the terminal tripeptide is probably not removed, and the C-terminus is not methylated.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5330411D17 antibody
    • 9830108K24Rik antibody
    • kinase PHKA1 antibody
    • KPB1 antibody
    • KPB1_HUMAN antibody
    • MGC132604 antibody
    • Pcyt1b antibody
    • PHKA antibody
    • PHKA1 antibody
    • Phosphorylase b kinase regulatory subunit alpha antibody
    • Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform antibody
    • Phosphorylase kinase alpha M subunit antibody
    • phosphorylase kinase, alpha 1 (muscle) antibody
    • RP23 210E20.1 antibody
    • skeletal muscle isoform antibody
    see all

Images

  • Anti-PHKA1 antibody (ab228593) at 1/500 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

    Predicted band size: 137 kDa



    5% SDS-PAGE gel.

  • Ice-cold methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PHKA1 (green) using ab228593 at 1/200 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

References

ab228593 has not yet been referenced specifically in any publications.

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