Recombinant
RabMAb

Anti-PHKA1 antibody [EPR12118] (ab176338)

Overview

  • Product name
    Anti-PHKA1 antibody [EPR12118]
    See all PHKA1 primary antibodies
  • Description
    Rabbit monoclonal [EPR12118] to PHKA1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PHKA1 aa 350-450 (Cysteine residue). The exact sequence is proprietary.
    Database link: P46020

  • Positive control
    • A431, Jurkat, HeLa, and Human skeletal muscle lysates. Paraffin-embedded Human skeletal muscle tissue. Permeabilized Hela cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab176338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 137 kDa.
IHC-P 1/100 - 1/250.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function
      Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin.
    • Tissue specificity
      Muscle specific. Isoform 1 is predominant in vastus lateralis muscle. Isoform 2 predominates slightly in heart, and it predominates clearly in the other tissues tested.
    • Pathway
      Glycan biosynthesis; glycogen metabolism.
    • Involvement in disease
      Glycogen storage disease 9D (GSD9D) [MIM:300559]: A metabolic disorder characterized by slowly progressive, predominantly distal muscle weakness and atrophy. Clinical features include exercise intolerance with early fatigability, pain, cramps and occasionally myoglobinuria. Note=The disease is caused by mutations affecting the gene represented in this entry.
    • Sequence similarities
      Belongs to the phosphorylase b kinase regulatory chain family.
    • Post-translational
      modifications
      Although the final Cys may be farnesylated, the terminal tripeptide is probably not removed, and the C-terminus is not methylated.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 5330411D17 antibody
      • 9830108K24Rik antibody
      • kinase PHKA1 antibody
      • KPB1 antibody
      • KPB1_HUMAN antibody
      • MGC132604 antibody
      • Pcyt1b antibody
      • PHKA antibody
      • PHKA1 antibody
      • Phosphorylase b kinase regulatory subunit alpha antibody
      • Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform antibody
      • Phosphorylase kinase alpha M subunit antibody
      • phosphorylase kinase, alpha 1 (muscle) antibody
      • RP23 210E20.1 antibody
      • skeletal muscle isoform antibody
      see all

    Images

    • Flow cytometric analysis of permeabilized Hela cells labeling PHKA1 with ab176338 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

    • Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling PHKA1 with ab176338 at 1/100 dilution.

    • All lanes : Anti-PHKA1 antibody [EPR12118] (ab176338) at 1/1000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Human skeletal muscle lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 137 kDa

    References

    ab176338 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab176338.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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