Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12118] to PHKA1
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Human
Product nameAnti-PHKA1 antibody [EPR12118]
See all PHKA1 primary antibodies
DescriptionRabbit monoclonal [EPR12118] to PHKA1
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC/IF or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human PHKA1 aa 350-450 (Cysteine residue). The exact sequence is proprietary.
Database link: P46020
- A431, Jurkat, HeLa, and Human skeletal muscle lysates. Paraffin-embedded Human skeletal muscle tissue. Permeabilized Hela cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab176338 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 137 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionPhosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin.
Tissue specificityMuscle specific. Isoform 1 is predominant in vastus lateralis muscle. Isoform 2 predominates slightly in heart, and it predominates clearly in the other tissues tested.
PathwayGlycan biosynthesis; glycogen metabolism.
Involvement in diseaseGlycogen storage disease 9D (GSD9D) [MIM:300559]: A metabolic disorder characterized by slowly progressive, predominantly distal muscle weakness and atrophy. Clinical features include exercise intolerance with early fatigability, pain, cramps and occasionally myoglobinuria. Note=The disease is caused by mutations affecting the gene represented in this entry.
Sequence similaritiesBelongs to the phosphorylase b kinase regulatory chain family.
modificationsAlthough the final Cys may be farnesylated, the terminal tripeptide is probably not removed, and the C-terminus is not methylated.
Cellular localizationCell membrane.
- Information by UniProt
- 5330411D17 antibody
- 9830108K24Rik antibody
- kinase PHKA1 antibody
All lanes : Anti-PHKA1 antibody [EPR12118] (ab176338) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Human skeletal muscle lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 137 kDa
Flow cytometric analysis of permeabilized Hela cells labeling PHKA1 with ab176338 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling PHKA1 with ab176338 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab176338 has been referenced in 2 publications.
- Liu X et al. Metformin and Berberine suppress glycogenolysis by inhibiting glycogen phosphorylase and stabilizing the molecular structure of glycogen in db/db mice. Carbohydr Polym 243:116435 (2020). PubMed: 32532388
- Krag TO et al. Differential glucose metabolism in mice and humans affected by McArdle disease. Am J Physiol Regul Integr Comp Physiol 311:R307-14 (2016). PubMed: 27280431