Recombinant Anti-PHKG1 antibody [EPR14812] (ab194112)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14812] to PHKG1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PHKG1 antibody [EPR14812]
See all PHKG1 primary antibodies -
Description
Rabbit monoclonal [EPR14812] to PHKG1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human skeletal muscle, mouse and rat muscle lysates; Human skeletal muscle, Human hepatocellular carcinoma and rat kidney tissues; A673 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14812 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab194112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 45 kDa).
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IHC-P |
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/100.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 45 kDa). |
IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/100. |
Target
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Function
Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. In vitro, phosphorylates PYGM, TNNI3, MAPT/TAU, GAP43 and NRGN/RC3. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 1 protein kinase domain. -
Domain
The two calmodulin-binding domains appear to act in concert to bind a single molecule of calmodulin and are pseudosubstrate/autoinhibitory domains. - Information by UniProt
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Database links
- Entrez Gene: 5260 Human
- Entrez Gene: 18682 Mouse
- Entrez Gene: 29353 Rat
- Omim: 172470 Human
- SwissProt: Q16816 Human
- SwissProt: P07934 Mouse
- SwissProt: P13286 Rat
- Unigene: 730821 Human
see all -
Alternative names
- PHK gamma M antibody
- PHK-gamma-M antibody
- Phkg antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling PHKG1 with ab194112 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Anti-PHKG1 antibody [EPR14812] (ab194112) at 1/20000 dilution + Human skeletal muscle tissue lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 45 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted? -
All lanes : Anti-PHKG1 antibody [EPR14812] (ab194112) at 1/1000 dilution
Lane 1 : Mouse muscle tissue lysate
Lane 2 : Rat muscle tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 45 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling PHKG1 with ab194112 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling PHKG1 with ab194112 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized A673 cells labeling PHKG1 with ab194112 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor®488) secondary antibody (ab150077) at 1/400 dilution. Nuclear counter stain is DAPI (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab194112 has been referenced in 1 publication.
- Liu X et al. Metformin and Berberine suppress glycogenolysis by inhibiting glycogen phosphorylase and stabilizing the molecular structure of glycogen in db/db mice. Carbohydr Polym 243:116435 (2020). PubMed: 32532388