Product nameAnti-PHKG2 antibody [EPR9727]
See all PHKG2 primary antibodies
DescriptionRabbit monoclonal [EPR9727] to PHKG2
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide corresponding to residues near the C-terminus of Human PHKG2 (UniProt: P15735)
- Human fetal lung, HepG2, Ramos, 293T and HeLa cell lysates. HepG2 cells, permeabilized 293T cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab167424 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 46 kDa.|
|ICC/IF||1/100 - 1/250.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
RelevanceMutations in PHKG2 along with PHKA2 and PHKB, all three different genes of phosphorylase kinase (Phk) subunits, can give rise to glycogen storage disease of the liver. The autosomal-recessive, liver-specific variant of Phk deficiency is caused by mutations in the gene encoding the testis/liver isoform of the catalytic gamma subunit, PHKG2.
- GSD9C antibody
- PHK gamma LT antibody
- PHK gamma T antibody
All lanes : Anti-PHKG2 antibody [EPR9727] (ab167424) at 1/1000 dilution
Lane 1 : Human fetal lung lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Ramos cell lysate
Lane 4 : 293T cell lysate
Lane 5 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP antibody at 1/2000 dilution
Predicted band size: 46 kDa
Immunofluorescent staining of HepG2 cells labeling PHKG2 with ab167424 at 1/100 dilution.
Flow cytometric analysis of permeabilized 293T cells labeling PHKG2 with ab167424 at 1/100 (red) or a rabbit IgG negative (green).
ab167424 has not yet been referenced specifically in any publications.