Overview

  • Product name

    Anti-PHLDA1 antibody [EPR6674]
    See all PHLDA1 primary antibodies
  • Description

    Rabbit monoclonal [EPR6674] to PHLDA1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within Human PHLDA1 (UniProt ID: Q8WV24).

  • Positive control

    • Human melanoma tissue lysate; A375 and BxPC3 cell lysates; Human colon carcinoma tissue; BxPC3 cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 45 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Seems to be involved in regulation of apoptosis. May be involved in detachment-mediated programmed cell death. May mediate apoptosis during neuronal development. May be involved in regulation of anti-apoptotic effects of IGF1. May be involved in translational regulation.
    • Tissue specificity

      Widely expressed with highest levels in pancreas. Strongly expressed by benign melanocytic nevi, and progressively reduced expressed in primary and metastatic melanomas (at protein level).
    • Sequence similarities

      Contains 1 PH domain.
    • Cellular localization

      Cytoplasm. Cytoplasmic vesicle. Nucleus, nucleolus. Colocalizes with intracellular vesicles.
    • Information by UniProt
    • Database links

    • Alternative names

      • Apoptosis associated nuclear protein antibody
      • Apoptosis-associated nuclear protein antibody
      • DT1P1B11 antibody
      • MGC131738 antibody
      • PHLA1_HUMAN antibody
      • PHLDA1 antibody
      • PHRIP antibody
      • Pleckstrin homology like domain family A member 1 antibody
      • Pleckstrin homology-like domain family A member 1 antibody
      • PQ rich protein antibody
      • PQ-rich protein antibody
      • PQR protein antibody
      • Proline- and glutamine-rich protein antibody
      • Proline- and histidine-rich protein antibody
      • T cell death associated gene antibody
      • T CELL DEATH ASSOCIATED GENE 51 antibody
      • T-cell death-associated gene 51 protein antibody
      • Tdag antibody
      • TDAG51 antibody
      see all

    Images

    • All lanes : Anti-PHLDA1 antibody [EPR6674] (ab133654) at 1/10000 dilution

      Lane 1 : Human melanoma tissue lysate
      Lane 2 : A375 cell lysate
      Lane 3 : BxPC3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 45 kDa

    • Immunocytochemical analysis of PHLDA1 in BxPC3 cells labelled with ab133654 at a 1/100 dilution.
    • Immunohistochemical analysis of PHLDA1 in paraffin embedded Human colon carcinoma tissue labelled with ab133654 at a 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Fearon AE  et al. PHLDA1 Mediates Drug Resistance in Receptor Tyrosine Kinase-Driven Cancer. Cell Rep 22:2469-2481 (2018). Read more (PubMed: 29490281) »
    • Necela BM  et al. The antineoplastic drug, trastuzumab, dysregulates metabolism in iPSC-derived cardiomyocytes. Clin Transl Med 6:5 (2017). Read more (PubMed: 28101782) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133654.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up