Recombinant
RabMAb

Anti-PHLDA1 antibody [EPR6674] (Alexa Fluor® 647) (ab207670)

Overview

  • Product name
    Anti-PHLDA1 antibody [EPR6674] (Alexa Fluor® 647)
    See all PHLDA1 primary antibodies
  • Description
    Rabbit monoclonal [EPR6674] to PHLDA1 (Alexa Fluor® 647)
  • Host species
    Rabbit
  • Conjugation
    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications
    Suitable for: ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PHLDA1 aa 350 to the C-terminus. The exact sequence is proprietary.
    Database link: Q8WV24

  • Positive control
    • ICC/IF: A375 cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer
    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol, 1% BSA, PBS
  • Concentration information loading...
  • Purity
    Affinity purified
  • Clonality
    Monoclonal
  • Clone number
    EPR6674
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab207670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000.

This product gave a positive signal in A375 cells fixed with 4% formaldehyde (10 min)

Target

  • Function
    Seems to be involved in regulation of apoptosis. May be involved in detachment-mediated programmed cell death. May mediate apoptosis during neuronal development. May be involved in regulation of anti-apoptotic effects of IGF1. May be involved in translational regulation.
  • Tissue specificity
    Widely expressed with highest levels in pancreas. Strongly expressed by benign melanocytic nevi, and progressively reduced expressed in primary and metastatic melanomas (at protein level).
  • Sequence similarities
    Contains 1 PH domain.
  • Cellular localization
    Cytoplasm. Cytoplasmic vesicle. Nucleus, nucleolus. Colocalizes with intracellular vesicles.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis associated nuclear protein antibody
    • Apoptosis-associated nuclear protein antibody
    • DT1P1B11 antibody
    • MGC131738 antibody
    • PHLA1_HUMAN antibody
    • PHLDA1 antibody
    • PHRIP antibody
    • Pleckstrin homology like domain family A member 1 antibody
    • Pleckstrin homology-like domain family A member 1 antibody
    • PQ rich protein antibody
    • PQ-rich protein antibody
    • PQR protein antibody
    • Proline- and glutamine-rich protein antibody
    • Proline- and histidine-rich protein antibody
    • T cell death associated gene antibody
    • T CELL DEATH ASSOCIATED GENE 51 antibody
    • T-cell death-associated gene 51 protein antibody
    • Tdag antibody
    • TDAG51 antibody
    see all

Images

  • ab207670 staining PHLDA1 in A375 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab207670 at 1/1000 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

ab207670 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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