Key features and details
- Rabbit polyclonal to PHLDA3
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PHLDA3 antibody
See all PHLDA3 primary antibodies
DescriptionRabbit polyclonal to PHLDA3
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide corresponding to Human PHLDA3 (internal sequence).
Database link: Q9Y5J5
- Human breast carcinoma tissue.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
PBS without Mg2+ and Ca2+.
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab196757 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Our Abpromise guarantee covers the use of ab196757 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
Functionp53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its directs transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May acts as a tumor suppressor.
Tissue specificityWidely expressed with lowest expression in liver and spleen.
Sequence similaritiesBelongs to the PHLDA3 family.
Contains 1 PH domain.
DomainThe PH domain binds phosphoinositides with a broad specificity. It competes with the PH domain of AKT1 and directly interferes with AKT1 binding to phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-triphosphate (PIP3), preventing AKT1 association to membrane lipids and subsequent activation of AKT1 signaling.
Cellular localizationCytoplasm. Membrane.
- Information by UniProt
- PHLA3_HUMAN antibody
- PHLDA 3 antibody
- phlda3 antibody
ab196757 has not yet been referenced specifically in any publications.