Overview

  • Product name

  • Description

    Rabbit polyclonal to PHLDA3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human PHLDA3 (internal sequence).
    Database link: Q9Y5J5

  • Positive control

    • Human breast carcinoma tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride

    PBS without Mg2+ and Ca2+.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab196757 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab196757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.

Target

  • Function

    p53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its directs transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May acts as a tumor suppressor.
  • Tissue specificity

    Widely expressed with lowest expression in liver and spleen.
  • Sequence similarities

    Belongs to the PHLDA3 family.
    Contains 1 PH domain.
  • Domain

    The PH domain binds phosphoinositides with a broad specificity. It competes with the PH domain of AKT1 and directly interferes with AKT1 binding to phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-triphosphate (PIP3), preventing AKT1 association to membrane lipids and subsequent activation of AKT1 signaling.
  • Cellular localization

    Cytoplasm. Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • PHLA3_HUMAN antibody
    • PHLDA 3 antibody
    • phlda3 antibody
    • Pleckstrin homology like domain family A member 2 antibody
    • Pleckstrin homology like domain family A member 3 antibody
    • Pleckstrin homology-like domain family A member 3 antibody
    • TDAG51/Ipl homolog 1 antibody
    • TDAG51/Ipl homologue 1 antibody
    • TIH 1 antibody
    • TIH1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling PHLDA3 with ab196757 at 1/50 dilution (left) and synthesized peptide (right).

References

ab196757 has not yet been referenced specifically in any publications.

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