Overview

  • Product name

  • Description

    Goat polyclonal to PHLDA3
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    RARQSLGTGTLVS

    , corresponding to C terminal amino acids 115-127 of Human PHLDA3.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab22822 was purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab22822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).

Target

  • Function

    p53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its directs transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May acts as a tumor suppressor.
  • Tissue specificity

    Widely expressed with lowest expression in liver and spleen.
  • Sequence similarities

    Belongs to the PHLDA3 family.
    Contains 1 PH domain.
  • Domain

    The PH domain binds phosphoinositides with a broad specificity. It competes with the PH domain of AKT1 and directly interferes with AKT1 binding to phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-triphosphate (PIP3), preventing AKT1 association to membrane lipids and subsequent activation of AKT1 signaling.
  • Cellular localization

    Cytoplasm. Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • PHLA3_HUMAN antibody
    • PHLDA 3 antibody
    • phlda3 antibody
    • Pleckstrin homology like domain family A member 2 antibody
    • Pleckstrin homology like domain family A member 3 antibody
    • Pleckstrin homology-like domain family A member 3 antibody
    • TDAG51/Ipl homolog 1 antibody
    • TDAG51/Ipl homologue 1 antibody
    • TIH 1 antibody
    • TIH1 antibody
    see all

Images

  • Lanes 1-2 : Anti-PHLDA3 antibody (ab22822) at 1 µg/ml
    Lane 3 : Anti-myc tag antibody at 1/1000 dilution

    Lanes 1 & 3 : HEK293 lysate overexpressing Human PHLDA3 in RIPA buffer at 10 µg
    Lane 2 : Mock-transfected HEK293

    Developed using the ECL technique.

    Predicted band size: 14 kDa

References

This product has been referenced in:

  • Leszczynska KB  et al. Hypoxia-induced p53 modulates both apoptosis and radiosensitivity via AKT. J Clin Invest 125:2385-98 (2015). Read more (PubMed: 25961455) »
  • Ohki R  et al. PHLDA3 is a novel tumor suppressor of pancreatic neuroendocrine tumors. Proc Natl Acad Sci U S A 111:E2404-13 (2014). Read more (PubMed: 24912192) »
See all 3 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (MDA-MB-468 cells)
Loading amount
20 µg
Specification
MDA-MB-468 cells
Gel Running Conditions
Reduced Denaturing (15 %)
Blocking step
Blocking One (Nakalai) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100%

理恵子 大木

Verified customer

Submitted Apr 14 2009

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