Anti-PHLDB2 antibody (ab234885)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-PHLDB2 antibody
See all PHLDB2 primary antibodies -
Description
Rabbit polyclonal to PHLDB2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human PHLDB2 aa 734-1030.
Database link: Q86SQ0 -
Positive control
- WB: Mouse liver lysate. IHC-P: Human adrenal gland and gastric cancer tissues. ICC/IF: HepG2 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab234885 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/5000. Predicted molecular weight: 142 kDa. | |
| IHC-P | 1/20 - 1/200. | |
| ICC/IF | 1/50 - 1/200. |
Target
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Function
Seems to be involved in the assembly of the postsynaptic apparatus. May play a role in acetyl-choline receptor (AChR) aggregation in the postsynaptic membrane. -
Sequence similarities
Contains 1 PH domain. -
Domain
The PH domain mediates the binding to phosphoinositides. -
Cellular localization
Cytoplasm. Membrane. Translocates to the plasma membrane at high levels of PtdIns(3,4,5)P3. At low levels of PtdIns(3,4,5)P3 is translocated to vesicular compartments. - Information by UniProt
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Database links
- Entrez Gene: 90102 Human
- Entrez Gene: 208177 Mouse
- Omim: 610298 Human
- SwissProt: Q86SQ0 Human
- SwissProt: Q8K1N2 Mouse
- Unigene: 603252 Human
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Alternative names
- LL5 beta antibody
- LL5B antibody
- LL5beta antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLDB2 antibody (ab234885)Paraffin-embedded human adrenal gland tissue stained for PHLDB2 using ab234885 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-PHLDB2 antibody (ab234885)HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling PHLDB2 using ab234885 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
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Western blot - Anti-PHLDB2 antibody (ab234885)Anti-PHLDB2 antibody (ab234885) at 1/500 dilution + Mouse liver lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 142 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLDB2 antibody (ab234885)Paraffin-embedded human gastric cancer tissue stained for PHLDB2 using ab234885 at 1/100 dilution in immunohistochemical analysis.
References
ab234885 has not yet been referenced specifically in any publications.
