Anti-PHLDB2 antibody (ab234885)
Key features and details
- Rabbit polyclonal to PHLDB2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PHLDB2 antibody
See all PHLDB2 primary antibodies -
Description
Rabbit polyclonal to PHLDB2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human PHLDB2 aa 734-1030.
Database link: Q86SQ0 -
Positive control
- WB: Mouse liver lysate. IHC-P: Human adrenal gland and gastric cancer tissues. ICC/IF: HepG2 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab234885 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/5000. Predicted molecular weight: 142 kDa. | |
| IHC-P | 1/20 - 1/200. | |
| ICC/IF | 1/50 - 1/200. |
Target
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Function
Seems to be involved in the assembly of the postsynaptic apparatus. May play a role in acetyl-choline receptor (AChR) aggregation in the postsynaptic membrane. -
Sequence similarities
Contains 1 PH domain. -
Domain
The PH domain mediates the binding to phosphoinositides. -
Cellular localization
Cytoplasm. Membrane. Translocates to the plasma membrane at high levels of PtdIns(3,4,5)P3. At low levels of PtdIns(3,4,5)P3 is translocated to vesicular compartments. - Information by UniProt
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Database links
- Entrez Gene: 90102 Human
- Entrez Gene: 208177 Mouse
- Omim: 610298 Human
- SwissProt: Q86SQ0 Human
- SwissProt: Q8K1N2 Mouse
- Unigene: 603252 Human
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Alternative names
- LL5 beta antibody
- LL5B antibody
- LL5beta antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLDB2 antibody (ab234885)
Paraffin-embedded human adrenal gland tissue stained for PHLDB2 using ab234885 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-PHLDB2 antibody (ab234885)HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling PHLDB2 using ab234885 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
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Western blot - Anti-PHLDB2 antibody (ab234885)Anti-PHLDB2 antibody (ab234885) at 1/500 dilution + Mouse liver lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 142 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLDB2 antibody (ab234885)Paraffin-embedded human gastric cancer tissue stained for PHLDB2 using ab234885 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab234885 has not yet been referenced specifically in any publications.
