Overview

  • Product name

  • Description

    Rabbit polyclonal to PHLPP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Guinea pig, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residues 1275 and the C-terminus (residue 1323) of human PHLPP2 (NP_055835.2)

  • Positive control

    • Whole cell lysates from HeLa, 293T or NIH/3T3 cells. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: A549

Properties

Applications

Our Abpromise guarantee covers the use of ab71973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/2000 - 1/10000. Detects a band of approximately 190 kDa (predicted molecular weight: 147 kDa).
IP Use at 1-4 µg/mg of lysate.

Target

  • Function

    Protein phosphatase that mediates dephosphorylation of 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor.
  • Tissue specificity

    In colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but is largely excluded from the crypt bases. Expression is lost or significantly decreased in 80% of tested tumors (at protein level).
  • Sequence similarities

    Contains 22 LRR (leucine-rich) repeats.
    Contains 1 PH domain.
    Contains 1 PP2C-like domain.
  • Cellular localization

    Cytoplasm. Membrane. Nucleus. In colorectal cancer tissue, expression is concentrated in the cytoplasm and nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • KIAA0931 antibody
    • PH domain and leucine rich repeat protein phosphatase 2 antibody
    • PH domain leucine rich repeat protein phosphatase like antibody
    • PH domain leucine-rich repeat-containing protein phosphatase 2 antibody
    • PH domain leucine-rich repeat-containing protein phosphatase-like antibody
    • PHLP2_HUMAN antibody
    • PHLPP like antibody
    • PHLPP-like antibody
    • Phlpp2 antibody
    • PHLPPL antibody
    see all

Images

  • All lanes : Anti-PHLPP2 antibody (ab71973) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Predicted band size: 147 kDa
    Observed band size: 190 kDa
    why is the actual band size different from the predicted?

  • Detection of PHLPP2 by Western Blot of Immunoprecipitate.
    ab71973 at 1µg/ml staining PHLPP2 in HeLa whole cell lysate immunoprecipitated using ab71973 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: chemiluminescence with exposure time of 30 seconds.
  • ab71973 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab71973 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Sun X  et al. MicroRNA-181b Improves Glucose Homeostasis and Insulin Sensitivity by Regulating Endothelial Function in White Adipose Tissue. Circ Res 118:810-21 (2016). Read more (PubMed: 26830849) »
  • Castellanos-Martín A  et al. Unraveling heterogeneous susceptibility and the evolution of breast cancer using a systems biology approach. Genome Biol 16:40 (2015). Read more (PubMed: 25853295) »
See all 3 Publications for this product

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