Overview

  • Product name

    Anti-PHLPP2 antibody - N-terminal
    See all PHLPP2 primary antibodies
  • Description

    Rabbit polyclonal to PHLPP2 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to a region within N terminal amino acids 13-234 of Human PHLPP2.

  • Positive control

    • Mouse brain whole cell lysate; A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab153918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 147 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Protein phosphatase that mediates dephosphorylation of 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor.
  • Tissue specificity

    In colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but is largely excluded from the crypt bases. Expression is lost or significantly decreased in 80% of tested tumors (at protein level).
  • Sequence similarities

    Contains 22 LRR (leucine-rich) repeats.
    Contains 1 PH domain.
    Contains 1 PP2C-like domain.
  • Cellular localization

    Cytoplasm. Membrane. Nucleus. In colorectal cancer tissue, expression is concentrated in the cytoplasm and nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • KIAA0931 antibody
    • PH domain and leucine rich repeat protein phosphatase 2 antibody
    • PH domain leucine rich repeat protein phosphatase like antibody
    • PH domain leucine-rich repeat-containing protein phosphatase 2 antibody
    • PH domain leucine-rich repeat-containing protein phosphatase-like antibody
    • PHLP2_HUMAN antibody
    • PHLPP like antibody
    • PHLPP-like antibody
    • Phlpp2 antibody
    • PHLPPL antibody
    see all

Images

  • Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling PHLPP2 with ab153918 at 1/500 dilution. Lower image is co-stained with Hoechst 33342.
  • Anti-PHLPP2 antibody - N-terminal (ab153918) at 1/2000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 147 kDa



    5% SDS PAGE

References

ab153918 has not yet been referenced specifically in any publications.

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