Overview

  • Product name

    Anti-PHLPP2 antibody [SP220]
    See all PHLPP2 primary antibodies
  • Description

    Rabbit monoclonal [SP220] to PHLPP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human PHLPP2 aa 1300 to the C-terminus. The exact sequence is proprietary.
    Database link: Q6ZVD8

  • Positive control

    • IHC-P: Human prostate, and prostate carcinoma tissue; WB: HEK293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab227673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/400. Predicted molecular weight: 147 kDa.

Primary antibody incubation for 1 hour at room temperature.

IHC-P 1/100.

Perform heat mediated antigen retrieval by boiling tissue section in citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Primary antibody incubation for 10 minutes at room temperature.

Target

  • Function

    Protein phosphatase that mediates dephosphorylation of 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor.
  • Tissue specificity

    In colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but is largely excluded from the crypt bases. Expression is lost or significantly decreased in 80% of tested tumors (at protein level).
  • Sequence similarities

    Contains 22 LRR (leucine-rich) repeats.
    Contains 1 PH domain.
    Contains 1 PP2C-like domain.
  • Cellular localization

    Cytoplasm. Membrane. Nucleus. In colorectal cancer tissue, expression is concentrated in the cytoplasm and nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • KIAA0931 antibody
    • PH domain and leucine rich repeat protein phosphatase 2 antibody
    • PH domain leucine rich repeat protein phosphatase like antibody
    • PH domain leucine-rich repeat-containing protein phosphatase 2 antibody
    • PH domain leucine-rich repeat-containing protein phosphatase-like antibody
    • PHLP2_HUMAN antibody
    • PHLPP like antibody
    • PHLPP-like antibody
    • Phlpp2 antibody
    • PHLPPL antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostate carcinoma tissue sections labeling PHLPP2 with ab227673 at 1/100 dilution (1.65 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Formalin-fixed, paraffin-embedded human prostate tissue stained for PHLPP2 using ab227673 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PHLPP2 using ab227673 at 1/100 dilution in immunohistochemical analysis.

  • Anti-PHLPP2 antibody [SP220] (ab227673) at 1/400 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate.

    Predicted band size: 147 kDa

References

ab227673 has not yet been referenced specifically in any publications.

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