Key features and details
- Mouse monoclonal [1D3A10] to PHO8
- Suitable for: WB, ICC/IF
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG1
Product nameAnti-PHO8 antibody [1D3A10]
DescriptionMouse monoclonal [1D3A10] to PHO8
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Saccharomyces cerevisiae
Full length native protein (purified). This information is considered to be commercially sensitive.
- Yeast lysate
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: HEPES buffered saline
Concentration information loading...
Purification notesNear homogeneity (Purity >95% by SDS-PAGE). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by chemical fractionation.
Light chain typekappa
Our Abpromise guarantee covers the use of ab113688 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 4 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).|
|ICC/IF||Use a concentration of 20 µg/ml.|
FunctionPhosphatase with broad substrate specificity. A truncated (soluble) version of the protein is responsible for the production of (E,E)-farnesol from (E,E)-farnesyl diphosphate.
Sequence similaritiesBelongs to the alkaline phosphatase family.
Cellular localizationCytoplasm. The truncated version of the protein is soluble and Vacuole membrane. The full-length version is found in lysosome-like vacuoles.
- Information by UniProt
- Farnesyl diphosphatase antibody
- Membrane-bound repressible alkaline phosphatase antibody
- PHO8 antibody
ab113688 has been referenced in 6 publications.
- Nguyen TQ et al. Isolated Saccharomyces cerevisiae vacuoles contain low-molecular-mass transition-metal polyphosphate complexes. Metallomics 11:1298-1309 (2019). PubMed: 31210222
- McNally EK & Brett CL The intralumenal fragment pathway mediates ESCRT-independent surface transporter down-regulation. Nat Commun 9:5358 (2018). PubMed: 30560896
- Szoradi T et al. SHRED Is a Regulatory Cascade that Reprograms Ubr1 Substrate Specificity for Enhanced Protein Quality Control during Stress. Mol Cell 70:1025-1037.e5 (2018). PubMed: 29861160
- Smindak RJ et al. Lipid-dependent regulation of exocytosis in S. cerevisiae by OSBP homolog (Osh) 4. J Cell Sci 130:3891-3906 (2017). PubMed: 28993464
- Manandhar SP et al. Saccharomyces cerevisiae Env7 is a novel serine/threonine kinase 16-related protein kinase and negatively regulates organelle fusion at the lysosomal vacuole. Mol Cell Biol 33:526-42 (2013). PubMed: 23166297
- Beach A et al. Mitochondrial membrane lipidome defines yeast longevity. Aging (Albany NY) 5:551-74 (2013). PubMed: 23924582