Overview

  • Product name

    Anti-PHO8 antibody [1D3A10]
  • Description

    Mouse monoclonal [1D3A10] to PHO8
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Full length native protein (purified). This information is considered to be commercially sensitive.

  • Positive control

    • Yeast lysate
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab113688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 4 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
ICC/IF Use a concentration of 20 µg/ml.

Target

  • Function

    Phosphatase with broad substrate specificity. A truncated (soluble) version of the protein is responsible for the production of (E,E)-farnesol from (E,E)-farnesyl diphosphate.
  • Sequence similarities

    Belongs to the alkaline phosphatase family.
  • Cellular localization

    Cytoplasm. The truncated version of the protein is soluble and Vacuole membrane. The full-length version is found in lysosome-like vacuoles.
  • Information by UniProt
  • Database links

    • Alternative names

      • Farnesyl diphosphatase antibody
      • Membrane-bound repressible alkaline phosphatase antibody
      • PHO8 antibody
      • PPB_YEAST antibody
      • Soluble alkaline phosphatase antibody
      see all

    Images

    • Anti-PHO8 antibody [1D3A10] (ab113688) + Yeast whole cell lysate at 15 µg

      Predicted band size: 63 kDa

    References

    This product has been referenced in:

    • McNally EK & Brett CL The intralumenal fragment pathway mediates ESCRT-independent surface transporter down-regulation. Nat Commun 9:5358 (2018). Read more (PubMed: 30560896) »
    • Manandhar SP  et al. Saccharomyces cerevisiae Env7 is a novel serine/threonine kinase 16-related protein kinase and negatively regulates organelle fusion at the lysosomal vacuole. Mol Cell Biol 33:526-42 (2013). Read more (PubMed: 23166297) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab113688.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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