Question (64072) | Phorbol 12-myristate 13-acetate (PMA), PKC activator (ab120297)

Go to datasheet (ab120297)

Question

Dear Sir/Madam, We would like to know if this Anti-CPI17 alpha (phospho T38) antibody has been detected in C2C12 cellular extracts. If so, could you please send me some information regarding the detecting with this antibody. Looking forward to hearing from you.

Answer

Thank you for contacting us.

We have not specifically tested ab52147 on C2C12 cells. However, we do have customer feedback regarding the use of this antibody to detect the phosphorylated protein in treated myometrial smooth muscle cells.

https://www.abcam.com/index.html?datasheet=52174&tab=abreviews&intabreviewid=13947

In his treatment, this customer used oxytocin, which we carry as ab120186 and phorbol 12-myristate 13-acetate (PMA), which we carry as ab120297.

https://www.abcam.com/Oxytocin-ab120186.html

https://www.abcam.com/Phorbol-12-myristate-13-acetate-PMA-ab120297.html

I do not know enough about the protein to tell you definitively whether or not this protein is endogenously phosphorylated in C2C12 cells. I do have an image of ab52174 used in WB with untreated RAW264.7 cells though, which we sell as ab7187. I'll be sure to get that WB image added to the datasheet online, but it will take a few days to appear.

https://www.abcam.com/RAW-264-7-Mouse-leukaemic-monocyte-macrophage-cell-line-Whole-Cell-Lysate-ab7187.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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