Key features and details
- Rabbit polyclonal to PHOS/PDC
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-PHOS/PDC antibody
See all PHOS/PDC primary antibodies
DescriptionRabbit polyclonal to PHOS/PDC
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Horse, Cow, Cat, Dog, Human
Synthetic peptide corresponding to Human PHOS/PDC aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following tissue lysates: Mouse Retina; Rat Retina; Rat Eye.
This product was previously labelled as PHOS
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab77523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).|
|IHC-P||Use a concentration of 1 µg/ml.|
FunctionMay participate in the regulation of visual phototransduction or in the integration of photoreceptor metabolism. Inhibits the transcriptional activation activity of the cone-rod homeobox CRX.
Sequence similaritiesBelongs to the phosducin family.
modificationsLight-induced changes in cyclic nucleotide levels modulate the phosphorylation of this protein by cAMP kinase.
Cellular localizationNucleus and Cytoplasm > cytosol. Cell projection > cilium > photoreceptor outer segment. Photoreceptor inner segment. Nucleus. Outer and inner segments of the rod cells.
- Information by UniProt
- 33 kDa phototransducing protein antibody
- MEKA protein antibody
- pdc antibody
All lanes : Anti-PHOS/PDC antibody (ab77523) at 1 µg/ml
Lane 1 : Mouse Retina Tissue Lysate
Lane 2 : Rat Retina Tissue Lysate
Lane 3 : Rat Eye Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 6 minutes
IHC image of ab77523 staining in Mouse Eye formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77523, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab77523 has been referenced in 1 publication.
- Lastowska M et al. Medulloblastoma with transitional features between Group 3 and Group 4 is associated with good prognosis. J Neurooncol 138:231-240 (2018). PubMed: 29427151