Recombinant Anti-PHOS/PDC antibody [EPR21913] (ab221138)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21913] to PHOS/PDC
- Suitable for: WB, IHC-P, IHC-Fr, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-PHOS/PDC antibody [EPR21913]
See all PHOS/PDC primary antibodies -
Description
Rabbit monoclonal [EPR21913] to PHOS/PDC -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IHC-Fr, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Human eye tissue lysate; Mouse and rat retina tissue lysate. IHC-P: Mouse, rat and human retina tissues. IHC-Fr: Mouse and rat retina tissues. IP: Mouse eyeball tissue lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21913 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab221138 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000. Predicted molecular weight: 28 kDa.
|
|
IHC-P |
1/2000.
Perform antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). |
|
IHC-Fr |
1/100.
Heat-mediated antigen retrieval by sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20) |
|
IP |
1/30.
|
Notes |
---|
WB
1/1000. Predicted molecular weight: 28 kDa. |
IHC-P
1/2000. Perform antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). |
IHC-Fr
1/100. Heat-mediated antigen retrieval by sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20) |
IP
1/30. |
Target
-
Function
May participate in the regulation of visual phototransduction or in the integration of photoreceptor metabolism. Inhibits the transcriptional activation activity of the cone-rod homeobox CRX. -
Sequence similarities
Belongs to the phosducin family. -
Post-translational
modificationsLight-induced changes in cyclic nucleotide levels modulate the phosphorylation of this protein by cAMP kinase. -
Cellular localization
Nucleus and Cytoplasm > cytosol. Cell projection > cilium > photoreceptor outer segment. Photoreceptor inner segment. Nucleus. Outer and inner segments of the rod cells. - Information by UniProt
-
Database links
- Entrez Gene: 5132 Human
- Entrez Gene: 20028 Mouse
- Entrez Gene: 25343 Rat
- Omim: 171490 Human
- SwissProt: P20941 Human
- SwissProt: Q9QW08 Mouse
- SwissProt: P20942 Rat
- Unigene: 654381 Human
see all -
Alternative names
- 33 kDa phototransducing protein antibody
- MEKA protein antibody
- pdc antibody
see all
Images
-
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized mouse retina tissue labeling PHOS/PDC with ab221138 at 1/100 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Positive staining on mouse retina is observed. Counterstained with DAPI (Nuclear).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.
-
All lanes : Anti-PHOS/PDC antibody [EPR21913] (ab221138) at 1/1000 dilution
Lane 1 : Human eye tissue lysate
Lane 2 : Mouse retina tissue lysate
Lane 3 : Rat retina tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 28 kDa
Exposure time: 15 secondsBlocking/diluting buffer and concentration: 5% NFDM/TBST.
The molecular mass observed is consistent with the literature (PMID 8816766).
-
Immunohistochemical analysis of paraffin-embedded mouse retina tissue labeling PHOS/PDC with ab221138 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Positive staining on mouse retina, performed on a Leica Biosystems BOND® RX instrument (PMID: 11287646, PMID: 10617777) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.Antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0).
-
Immunohistochemical analysis of paraffin-embedded rat retina tissue labeling PHOS/PDC with ab221138 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Positive staining on rat retina, performed on a Leica Biosystems BOND® RX instrument (PMID: 11287646, PMID: 10617777) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.Antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0).
-
Immunohistochemical analysis of paraffin-embedded human retina tissue labeling PHOS/PDC with ab221138 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Positive staining on human retina, performed on a Leica Biosystems BOND® RX instrument (PMID: 11287646, PMID: 10617777, PMID: 15842737) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.
Antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0).
-
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized rat retina tissue labeling PHOS/PDC with ab221138 at 1/100 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Positive staining on mouse retina is observed. Counterstained with DAPI (Nuclear).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.
-
PHOS/PDC was immunoprecipitated from 0.35 mg of mouse eyeball tissue lysate with ab221138 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221138 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.
Lane 1: Mouse eyeball tissue lysate 10 μg (input).
Lane 2: ab221138 IP in mouse eyeball tissue lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab221138 in mouse eyeball lysate.Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (0)
ab221138 has not yet been referenced specifically in any publications.