Overview

  • Product name

    Anti-PHOS/PDC antibody [EPR21913]
    See all PHOS/PDC primary antibodies
  • Description

    Rabbit monoclonal [EPR21913] to PHOS/PDC
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein within Human PHOS/PDC aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P20941

  • Positive control

    • WB: Human eye tissue lysate; Mouse and rat retina tissue lysate. IHC-P: Mouse, rat and human retina tissues. IHC-Fr: Mouse and rat retina tissues. IP: Mouse eyeball tissue lysate.
  • General notes

     This product was previously labelled as PHOS

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR21913
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab221138 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 28 kDa.
IHC-P 1/2000.

Perform antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).

IHC-Fr 1/100.

Heat-mediated antigen retrieval by sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20)

IP 1/30.

Target

  • Function

    May participate in the regulation of visual phototransduction or in the integration of photoreceptor metabolism. Inhibits the transcriptional activation activity of the cone-rod homeobox CRX.
  • Sequence similarities

    Belongs to the phosducin family.
  • Post-translational
    modifications

    Light-induced changes in cyclic nucleotide levels modulate the phosphorylation of this protein by cAMP kinase.
  • Cellular localization

    Nucleus and Cytoplasm > cytosol. Cell projection > cilium > photoreceptor outer segment. Photoreceptor inner segment. Nucleus. Outer and inner segments of the rod cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • 33 kDa phototransducing protein antibody
    • MEKA protein antibody
    • pdc antibody
    • PHD antibody
    • PHOS_HUMAN antibody
    • Phosducin antibody
    • Protein MEKA antibody
    see all

Images

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized mouse retina tissue labeling PHOS/PDC with ab221138 at 1/100 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Positive staining on mouse retina is observed. Counterstained with DAPI (Nuclear).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

  • All lanes : Anti-PHOS/PDC antibody [EPR21913] (ab221138) at 1/1000 dilution

    Lane 1 : Human eye tissue lysate
    Lane 2 : Mouse retina tissue lysate
    Lane 3 : Rat retina tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 28 kDa


    Exposure time: 15 seconds


    Blocking/diluting buffer and concentration: 5% NFDM/TBST.

    The molecular mass observed is consistent with the literature (PMID 8816766).

  • Immunohistochemical analysis of paraffin-embedded mouse retina tissue labeling PHOS/PDC with ab221138 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Positive staining on mouse retina, performed on a Leica Biosystems BOND® RX instrument (PMID: 11287646, PMID: 10617777) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.

    Antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0).

  • Immunohistochemical analysis of paraffin-embedded rat retina tissue labeling PHOS/PDC with ab221138 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Positive staining on rat retina, performed on a Leica Biosystems BOND® RX instrument (PMID: 11287646, PMID: 10617777) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.

    Antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0).

  • Immunohistochemical analysis of paraffin-embedded human retina tissue labeling PHOS/PDC with ab221138 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Positive staining on human retina, performed on a Leica Biosystems BOND® RX instrument (PMID: 11287646, PMID: 10617777, PMID: 15842737) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.

    Antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0).

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized rat retina tissue labeling PHOS/PDC with ab221138 at 1/100 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Positive staining on mouse retina is observed. Counterstained with DAPI (Nuclear).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

  • PHOS/PDC was immunoprecipitated from 0.35 mg of mouse eyeball tissue lysate with ab221138 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221138 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.
    Lane 1: Mouse eyeball tissue lysate 10 μg (input).
    Lane 2: ab221138 IP in mouse eyeball tissue lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab221138 in mouse eyeball lysate.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

References

ab221138 has not yet been referenced specifically in any publications.

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