Product namePhosphate Assay Kit - PiColorLockTM
Phosphate Assay Kit- PiColorLockTM (ab270004) utilizes a specially formulated reagent to give sensitive detection of inorganic phosphates (Pi) and provides an alternative to the more hazardous radioactive methods and other less sensitive colorimetric assays.
This product is manufactured by Expedeon, an Abcam company. It was previously called PiColorLock. Expedeon product code 303-0030 is the same as the 625 test size of this product and code 303-0125 is the same as the 2500 test size.
The reagent is compatible with DMSO, the solvent most commonly used in high throughput screening (HTS) applications.
The PiColorLock™ assay is based on the change in absorbance of the dye malachite green in the presence of phosphomolybdate complexes. Unlike most malachite dye-based solutions, PiColorLock gives a stable end- point signal and is not prone to precipitation. Moreover, a special stabilizer ensures that the reagent can be used with acid labile substrates.
The kit comes in 2 sizes:
- 625 test (1560 assays)
- 2500 test (6250 assays)
Tested applicationsSuitable for: Functional Studiesmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 625 tests 2500 tests 0.1mM Pi standard 1 unit 1 unit Accelerator 1 vial 2 vials PiColorLock 1 x 30ml 1 x 125ml Stabiliser 1 unit 1 unit
Our Abpromise guarantee covers the use of ab270004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ATP has been incubated in three detection reagents. A steadily rising background signal is seen with competitor reagents, whereas PiColorLock™ provides baseline readings.
PiColorLock™ has also been designed to have a large linear range, thus reducing the need for sample dilution. Competitors’ products are linear over a much narrower range of concentrations.
ab270004 has not yet been referenced specifically in any publications.