Product namePhosphate Buffered Saline (PBS) Casein ELISA Reagent
See all ELISA buffer reagents
Tested applicationsSuitable for: ELISAmore details
Unsuitable for: IHC-Fr or IHC-P
Abcam’s Phosphate Buffered Saline (PBS) Casein ELISA Reagent consists of many phosphoserine groups that function to bind Ca2+. The solution is ready for dilution to specific application requirements. ab171532 contributes to the minimization of non-specific interactions of the secondary reagents with each other and with the primary solid phase binding sites. The high blocking efficiency of casein is due to its content of small molecular weight proteins. Phosphate Buffered Saline (PBS), pH 7.4, is a poor buffer above pH 7.5. Phosphate buffer should not be used in assays where competition for phosphate groups or complex formation with a metal ion is essential for the enzyme activation. Phosphate ions will inhibit carboxypeptidiase, carboxylase, urease, muscle diaminase, formase and phosphoglucomutase.
This product should be clear and colorless in appearance. To minimize the risk of contamination, a fresh working solution should be prepared each day or store unused working solutions at the recommended storage temperature. If proper technique is not performed the possibility of introducing contaminants is always possible.
ab171532 should be diluted with reagent quality water. To produce a 1X working solution, dilute 1 mL of Phosphate Buffered Saline (PBS) Casein ELISA Reagent in 4 mL reagent quality water. To use as a blocking reagent, treat target surface by covering completely with 1X solution. To use as a diluent, dilute antigen/antibody to desired concentration in 1X solution and treat surface by covering completely. When using 1X solution for coating plates, best results are obtained following an overnight incubation at +4°C.
Storage instructionsStore at room temperature.
Storage bufferpH: 7
Concentration information loading...
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Tris Buffered Saline (TBS) Casein ELISA Reagent (ab171533)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
- AP Stability Conjugate Reagent (ab171536)
- HRP Stability Conjugate Reagent (ab171537)
Our Abpromise guarantee covers the use of ab171532 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab171532 has not yet been referenced specifically in any publications.