Anti-Phospho - (Ser/Thr) Phe antibody (ab17464)

Abreviews (5)Q&A (14)Specific References (33)

Overview

  • Product name
    Anti-Phospho - (Ser/Thr) Phe antibody
  • Description
    Rabbit polyclonal to Phospho - (Ser/Thr) Phe
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, ELISA, IP, WBmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Synthetic peptide corresponding to Phospho - (Ser/Thr) Phe . Immunogen = Synthetic phospho-serine/threonine-phenylalanine containing peptide.

  • Positive control
    • Calyculin A treated A431 cells
  • General notes

    The immunogen used is specific phosphorylated serine or threonine amino acids surrounded by phenylalanine. (Y/F/W)(S*/T*)F

Properties

Applications

Our Abpromise guarantee covers the use of ab17464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
ELISA 1/1000.
IP 1/100.
WB 1/1000.

Block with 5% nonfat milk, 0.1% Tween-20. Dilute primary antibody in 5% BSA, 0.1% Tween-20.

Images

  • Western blot - Anti-Phospho - (Ser/Thr) Phe antibody (ab17464)
    Western blot - Anti-Phospho - (Ser/Thr) Phe antibody (ab17464)Image from Gao X et al, PLoS Pathog 5:e1000708 (2009), Fig S3.
    To determine if NleH could phosphorylate RPS3, we conducted in vitro kinase assays with NleH and RPR3 and analyzed the results by Immunoblotting with ab17464, phospho-Ser/Thr-specific antibody, following separation by SDS-PAGE.
  • Western blot - Anti-Phospho - (Ser/Thr) Phe antibody (ab17464)
    Western blot - Anti-Phospho - (Ser/Thr) Phe antibody (ab17464)This image is courtesy of an anonymous Abreview
    All lanes : Anti-Phospho - (Ser/Thr) Phe antibody (ab17464) at 1/1000 dilution

    Lane 1 : Human THP-1 Whole Cell Extract
    Lane 2 : Mouse Total Lung Extract

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Alkaline phosphatase conjugated goat anti-rabbit antibody

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 5 minutes

    See Abreview

  • Western blot - Anti-Phospho - (Ser/Thr) Phe antibody (ab17464)
    Western blot - Anti-Phospho - (Ser/Thr) Phe antibody (ab17464)
    All lanes : Anti-Phospho - (Ser/Thr) Phe antibody (ab17464) at 1/1000 dilution

    All lanes : calyculin A treated A431 cells

References

This product has been referenced in:
  • Wei X  et al. Targeting the Dvl-1/ß-arrestin2/JNK3 interaction disrupts Wnt5a-JNK3 signaling and protects hippocampal CA1 neurons during cerebral ischemia reperfusion. Neuropharmacology 135:11-21 (2018). IP ; Rat . Read more (PubMed: 29510185) »
  • Po A  et al. Noncanonical GLI1 signaling promotes stemness features and in vivo growth in lung adenocarcinoma. Oncogene 36:4641-4652 (2017). Read more (PubMed: 28368412) »

See all 33 Publications for this product

Publishing research using ab17464? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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19 reviews or Q&A

10
Votes: Highest First

Western blot abreview for Anti-Phosphoserine/threonine antibody

 Good
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (hepatocytes)
Specification
hepatocytes
Treatment
20 ug/ml poly(I:C)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Abcam user community

Verified customer

Submitted Jun 26 2014

Western blot abreview for Anti-Phosphoserine/threonine antibody

 Below Average
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
100 µg
Specification
HeLa
Treatment
UV irradiation
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted May 13 2013

We wanted to check the general phosphorylated serine/threonine level of our protein (especially for phospho-threonine). So we would like to have a antibody that could specific recognize all phosphorylated serine/threonine residues (even p-Tyr), but not...

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Thank you for contacting us.

The ab9337 recognizes proteins phosphorylated on threonine residues. It does not cross-react with phosphotyrosine.

For ab15556, this antibody recognizes serine, threonine, and tyrosine phosphorylated pr...

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we would appreciate if we should have the opportunity to test instead the ab17464 antibody, the ab15556 antibody.
Many thanks and best regards

Thank you very much for your email.
The antibody ab17464 has been qc tested for its specificity and we have found no problem with it. It is highly likely that the expressed protein you are using is not compatible with the antibodies, therefore I wo...

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referring to our recent inquiry for the antibody anti phospho
serine/threonine (cat n° 17464), the new lot of antibody was tested.
Unfortunately, neither the new lot worked, the results was no band
detected, not even in the positive ...

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Thank you for contacting us. I am sorry to hear this antibody is not providing satisfactory results.
The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I apprecia...

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Abcam customer care service is really helpful and cooperative. We really
appreciate and give thanks for your help.
I prefer a replacement with a different lot. I will inform you as soon as
the new lot is checked.
Regards

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

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as suggested from you in the previous email, a new test of the antibody
(code ab17464) was checked and the phosphorylation of the recombinant
protein was assessed by performing incubation with CHIP.

Answering to your questions, the...

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Thank you for your cooperation in this case.
It may well be that this antibody in vial might have degraded or might have lost the specificity due to unknown reasons. In order to avoid any further delays in your project I am happy to offer you eith...

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in attaching you can find the filled questionnaire, and the immuno blotting film.
Waiting for instructions from you
My best regards

Thank you for your email. I am sorry to hear that you are experiencing problems with this antibody.
I have checked the protocol which seems fine to me. However I have doubt about the recombinant proteins you have been using. Could you please give ...

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I am writing you in reference to our recent order (n. 247 / 2012), of the anti-Phosphoserine / Threonine antibody (cat n. 17464).
Yesterday (May 3th), we tried the antibody.
Unfortunately, after tree tests, we didn't obtain any signal.
F...

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Thank you for contacting us.
I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
I am attaching ...

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Immunocytochemistry/ Immunofluorescence abreview for Anti-Phosphoserine/threonine antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human osteosarcoma SaOS-2)
Specification
human osteosarcoma SaOS-2
Fixative
Paraformaldehyde
Permeabilization
Yes - PBS TRITON .25%
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
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Submitted May 02 2012

1-10 of 19 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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