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Signal Transduction Metabolism Amino Acids
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Anti-Phospho - (Ser/Thr) Phe antibody (ab17464)

  • Datasheet
  • SDS
Reviews (6)Q&A (14)References (56)

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Western blot - Anti-Phospho - (Ser/Thr) Phe antibody (ab17464)
  • Western blot - Anti-Phospho - (Ser/Thr) Phe antibody (ab17464)
  • Western blot - Anti-Phospho - (Ser/Thr) Phe antibody (ab17464)

Key features and details

  • Rabbit polyclonal to Phospho - (Ser/Thr) Phe
  • Suitable for: ELISA, IP, WB
  • Reacts with: Species independent
  • Isotype: IgG

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Overview

  • Product name

    Anti-Phospho - (Ser/Thr) Phe antibody
  • Description

    Rabbit polyclonal to Phospho - (Ser/Thr) Phe
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ELISA, IP, WBmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide corresponding to Phospho - (Ser/Thr) Phe . Immunogen = Synthetic phospho-serine/threonine-phenylalanine containing peptide.

  • General notes

    The immunogen used is specific phosphorylated serine or threonine amino acids surrounded by phenylalanine. (Y/F/W)(S*/T*)F

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.50
    Constituents: 0.238% HEPES, 50% Glycerol, 0.87% Sodium chloride, 0.01% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified by Protein A and peptide affinity chromatography.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Regulation
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa Ser/Thr Phosphatase-Inhibited Whole Cell Lysate Set: Calyculin-Treated and Vehicle-Treated (ab135395)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab17464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
1/1000.
IP
1/100.
WB (5)
1/1000.

Block with 5% nonfat milk, 0.1% Tween-20. Dilute primary antibody in 5% BSA, 0.1% Tween-20.

Notes
ELISA
1/1000.
IP
1/100.
WB
1/1000.

Block with 5% nonfat milk, 0.1% Tween-20. Dilute primary antibody in 5% BSA, 0.1% Tween-20.

Images

  • Western blot - Anti-Phospho - (Ser/Thr) Phe antibody (ab17464)
    Western blot - Anti-Phospho - (Ser/Thr) Phe antibody (ab17464)Image from Gao X et al, PLoS Pathog 5:e1000708 (2009), Fig S3.
    To determine if NleH could phosphorylate RPS3, we conducted in vitro kinase assays with NleH and RPR3 and analyzed the results by Immunoblotting with ab17464, phospho-Ser/Thr-specific antibody, following separation by SDS-PAGE.
  • Western blot - Anti-Phospho - (Ser/Thr) Phe antibody (ab17464)
    Western blot - Anti-Phospho - (Ser/Thr) Phe antibody (ab17464)This image is courtesy of an anonymous Abreview
    All lanes : Anti-Phospho - (Ser/Thr) Phe antibody (ab17464) at 1/1000 dilution

    Lane 1 : Human THP-1 Whole Cell Extract
    Lane 2 : Mouse Total Lung Extract

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Alkaline phosphatase conjugated goat anti-rabbit antibody

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 5 minutes

    See Abreview

  • Western blot - Anti-Phospho - (Ser/Thr) Phe antibody (ab17464)
    Western blot - Anti-Phospho - (Ser/Thr) Phe antibody (ab17464)
    All lanes : Anti-Phospho - (Ser/Thr) Phe antibody (ab17464) at 1/1000 dilution

    All lanes : calyculin A treated A431 cells

Protocols

  • Immunoprecipitation protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (56)

    Publishing research using ab17464? Please let us know so that we can cite the reference in this datasheet.

    ab17464 has been referenced in 56 publications.

    • Feng X  et al. Ubiquitination of UVRAG by SMURF1 promotes autophagosome maturation and inhibits hepatocellular carcinoma growth. Autophagy 15:1130-1149 (2019). PubMed: 30686098
    • Guo X  et al. Inhibition of the Akt1-mTORC1 Axis Alters Venous Remodeling to Improve Arteriovenous Fistula Patency. Sci Rep 9:11046 (2019). PubMed: 31363142
    • Dong H  et al. GLI1 activation by non-classical pathway integrin avß3/ERK1/2 maintains stem cell-like phenotype of multicellular aggregates in gastric cancer peritoneal metastasis. Cell Death Dis 10:574 (2019). PubMed: 31366904
    • Akoume MY  et al. A Differential Hypofunctionality of Gai Proteins Occurs in Adolescent Idiopathic Scoliosis and Correlates with the Risk of Disease Progression. Sci Rep 9:10074 (2019). PubMed: 31296888
    • Cohen TS  et al. Staphylococcus aureus drives expansion of low-density neutrophils in diabetic mice. J Clin Invest 129:2133-2144 (2019). PubMed: 30985291
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-5 of 5 Abreviews or Q&A

    Western blot abreview for Anti-Phospho - (Ser/Thr) Phe antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Mouse liver)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    18 µg
    Specification
    Mouse liver
    Blocking step
    BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 15 2018

    Western blot abreview for Anti-Phosphoserine/threonine antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Mouse Cell lysate - whole cell (hepatocytes)
    Specification
    hepatocytes
    Treatment
    20 ug/ml poly(I:C)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 26 2014

    Western blot abreview for Anti-Phosphoserine/threonine antibody

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Loading amount
    100 µg
    Specification
    HeLa
    Treatment
    UV irradiation
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 13 2013

    Western blot abreview for Anti-Phosphoserine/threonine antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Lung)
    Loading amount
    10 µg
    Specification
    Lung
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 28 2007

    Western blot abreview for Anti-Phosphoserine/threonine antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (THP-1 cells)
    Loading amount
    10 µg
    Specification
    THP-1 cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 24 2007

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