Anti-Phospho - (Ser/Thr) Phe antibody (ab17464)
Key features and details
- Rabbit polyclonal to Phospho - (Ser/Thr) Phe
- Suitable for: ELISA, IP, WB
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-Phospho - (Ser/Thr) Phe antibody -
Description
Rabbit polyclonal to Phospho - (Ser/Thr) Phe -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, IP, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to Phospho - (Ser/Thr) Phe . Immunogen = Synthetic phospho-serine/threonine-phenylalanine containing peptide.
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General notes
The immunogen used is specific phosphorylated serine or threonine amino acids surrounded by phenylalanine. (Y/F/W)(S*/T*)F
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.50
Constituents: 0.238% HEPES, 50% Glycerol, 0.87% Sodium chloride, 0.01% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified by Protein A and peptide affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab17464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
1/1000.
|
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| IP |
1/100.
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|
| WB | (5) |
1/1000.
Block with 5% nonfat milk, 0.1% Tween-20. Dilute primary antibody in 5% BSA, 0.1% Tween-20. |
| Notes |
|---|
|
ELISA
1/1000. |
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IP
1/100. |
|
WB
1/1000. Block with 5% nonfat milk, 0.1% Tween-20. Dilute primary antibody in 5% BSA, 0.1% Tween-20. |
Images
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Western blot - Anti-Phospho - (Ser/Thr) Phe antibody (ab17464)Image from Gao X et al, PLoS Pathog 5:e1000708 (2009), Fig S3.To determine if NleH could phosphorylate RPS3, we conducted in vitro kinase assays with NleH and RPR3 and analyzed the results by Immunoblotting with ab17464, phospho-Ser/Thr-specific antibody, following separation by SDS-PAGE.
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Western blot - Anti-Phospho - (Ser/Thr) Phe antibody (ab17464)This image is courtesy of an anonymous AbreviewAll lanes : Anti-Phospho - (Ser/Thr) Phe antibody (ab17464) at 1/1000 dilution
Lane 1 : Human THP-1 Whole Cell Extract
Lane 2 : Mouse Total Lung Extract
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Alkaline phosphatase conjugated goat anti-rabbit antibody
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 5 minutes
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Western blot - Anti-Phospho - (Ser/Thr) Phe antibody (ab17464)All lanes : Anti-Phospho - (Ser/Thr) Phe antibody (ab17464) at 1/1000 dilution
All lanes : calyculin A treated A431 cells
Protocols
References (56)
ab17464 has been referenced in 56 publications.
- Feng X et al. Ubiquitination of UVRAG by SMURF1 promotes autophagosome maturation and inhibits hepatocellular carcinoma growth. Autophagy 15:1130-1149 (2019). PubMed: 30686098
- Guo X et al. Inhibition of the Akt1-mTORC1 Axis Alters Venous Remodeling to Improve Arteriovenous Fistula Patency. Sci Rep 9:11046 (2019). PubMed: 31363142
- Dong H et al. GLI1 activation by non-classical pathway integrin avß3/ERK1/2 maintains stem cell-like phenotype of multicellular aggregates in gastric cancer peritoneal metastasis. Cell Death Dis 10:574 (2019). PubMed: 31366904
- Akoume MY et al. A Differential Hypofunctionality of Gai Proteins Occurs in Adolescent Idiopathic Scoliosis and Correlates with the Risk of Disease Progression. Sci Rep 9:10074 (2019). PubMed: 31296888
- Cohen TS et al. Staphylococcus aureus drives expansion of low-density neutrophils in diabetic mice. J Clin Invest 129:2133-2144 (2019). PubMed: 30985291
