Anti-Phospho - (Ser/Thr) Phe antibody (ab17464)
Key features and details
- Rabbit polyclonal to Phospho - (Ser/Thr) Phe
- Suitable for: ELISA, IP, WB, ICC/IF
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-Phospho - (Ser/Thr) Phe antibody
See all Phospho - (Ser/Thr) Phe primary antibodies -
Description
Rabbit polyclonal to Phospho - (Ser/Thr) Phe -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, IP, WB, ICC/IFmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to Phospho - (Ser/Thr) Phe . Immunogen = Synthetic phospho-serine/threonine-phenylalanine containing peptide.
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General notes
The immunogen used is specific phosphorylated serine or threonine amino acids surrounded by phenylalanine. (Y/F/W)(S*/T*)F
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.50
Constituents: 0.238% HEPES, 50% Glycerol, 0.87% Sodium chloride, 0.01% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified by Protein A and peptide affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab17464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
1/1000.
|
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| IP |
1/100.
|
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| WB | (5) |
1/1000.
Block with 5% nonfat milk, 0.1% Tween-20. Dilute primary antibody in 5% BSA, 0.1% Tween-20. |
| ICC/IF | (1) |
Use at an assay dependent concentration.
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| Notes |
|---|
|
ELISA
1/1000. |
|
IP
1/100. |
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WB
1/1000. Block with 5% nonfat milk, 0.1% Tween-20. Dilute primary antibody in 5% BSA, 0.1% Tween-20. |
|
ICC/IF
Use at an assay dependent concentration. |
Images
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Western blot - Anti-Phospho - (Ser/Thr) Phe antibody (ab17464)Image from Gao X et al, PLoS Pathog 5:e1000708 (2009), Fig S3.To determine if NleH could phosphorylate RPS3, we conducted in vitro kinase assays with NleH and RPR3 and analyzed the results by Immunoblotting with ab17464, phospho-Ser/Thr-specific antibody, following separation by SDS-PAGE.
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Western blot - Anti-Phospho - (Ser/Thr) Phe antibody (ab17464)This image is courtesy of an anonymous AbreviewAll lanes : Anti-Phospho - (Ser/Thr) Phe antibody (ab17464) at 1/1000 dilution
Lane 1 : Human THP-1 Whole Cell Extract
Lane 2 : Mouse Total Lung Extract
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Alkaline phosphatase conjugated goat anti-rabbit antibody
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 5 minutes
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Western blot - Anti-Phospho - (Ser/Thr) Phe antibody (ab17464)All lanes : Anti-Phospho - (Ser/Thr) Phe antibody (ab17464) at 1/1000 dilution
All lanes : calyculin A treated A431 cells
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (79)
ab17464 has been referenced in 79 publications.
- Xie M et al. Sublytic C5b-9 induces glomerular mesangial cell proliferation via ERK1/2-dependent SOX9 phosphorylation and acetylation by enhancing Cyclin D1 in rat Thy-1 nephritis. Exp Mol Med 53:572-590 (2021). PubMed: 33811247
- De Luca E et al. Protein kinase Ca regulates the nucleocytoplasmic shuttling of KRIT1. J Cell Sci 134:N/A (2021). PubMed: 33443102
- Zuliani I et al. The Dysregulation of OGT/OGA Cycle Mediates Tau and APP Neuropathology in Down Syndrome. Neurotherapeutics 18:340-363 (2021). PubMed: 33258073
- Dekel E et al. 20S proteasomes secreted by the malaria parasite promote its growth. Nat Commun 12:1172 (2021). PubMed: 33608523
- Zhou L et al. NEK2 Promotes Cell Proliferation and Glycolysis by Regulating PKM2 Abundance via Phosphorylation in Diffuse Large B-Cell Lymphoma. Front Oncol 11:677763 (2021). PubMed: 34168996