Overview

  • Product name

    Anti-Phospho SQ/TQ ATM/ATR Substrate antibody
  • Description

    Rabbit polyclonal to Phospho SQ/TQ ATM/ATR Substrate
  • Host species

    Rabbit
  • Specificity

    Ab130947 recognizes proteins phosphorylated on SQ/TQ motifs (ATM/ATR kinase consensus phosphorylation site motif)
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Phospho SQ/TQ ATM/ATR Substrate aa 2600-2700 (phospho S2609 + T2609) conjugated to keyhole limpet haemocyanin.
    Database link: P78527

  • Positive control

    • This antibody gave a positive signal in Western Blot in the following cell line: HeLa untreated and UV treated whole cell

Properties

Applications

Our Abpromise guarantee covers the use of ab130947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 450 kDa (predicted molecular weight: 469 kDa).

An increase in signal is observed upon UV treatment

Target

  • Relevance

    Several protein kinases mediate cellular responses to DNA damage. This includes the serine/threonine kinases ATM (ataxia telangiectasia mutated) and ATR (ATM and Rad3-related). In response to DNA damage ATM and ATR phosphorylate a large range of proteins on Serine (S) or Threonine (T) residues next to a Glutamine (Q) residue, so called SQ or TQ consensus sites.
  • Alternative names

    • ATM antibody
    • ATR antibody
    • DNA PK antibody
    • DNAPK antibody
    • phospho S/T-Q antibody
    • phospho SQ/TQ antibody
    • PI3K antibody
    • PIKK antibody
    • pS/T-Q antibody
    • pSQ/TQ antibody
    • SQ/TQ antibody
    see all

Images

  • All lanes : Anti-Phospho SQ/TQ ATM/ATR Substrate antibody (ab130947) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Hela Whole Cell Lysate - UV Irradiated

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 469 kDa
    Observed band size: 450 kDa
    why is the actual band size different from the predicted?


    Exposure time: 8 minutes


    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab130947 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab130947 has not yet been referenced specifically in any publications.

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