Key features and details
- Rabbit polyclonal to Phospho SQ/TQ ATM/ATR Substrate
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Phospho SQ/TQ ATM/ATR Substrate antibody
DescriptionRabbit polyclonal to Phospho SQ/TQ ATM/ATR Substrate
SpecificityAb130947 recognizes proteins phosphorylated on SQ/TQ motifs (ATM/ATR kinase consensus phosphorylation site motif)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human Phospho SQ/TQ ATM/ATR Substrate aa 2600-2700 (phospho S2609 + T2609) conjugated to keyhole limpet haemocyanin.
Database link: P78527
- This antibody gave a positive signal in Western Blot in the following cell line: HeLa untreated and UV treated whole cell
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab130947 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 450 kDa (predicted molecular weight: 469 kDa).
An increase in signal is observed upon UV treatment
RelevanceSeveral protein kinases mediate cellular responses to DNA damage. This includes the serine/threonine kinases ATM (ataxia telangiectasia mutated) and ATR (ATM and Rad3-related). In response to DNA damage ATM and ATR phosphorylate a large range of proteins on Serine (S) or Threonine (T) residues next to a Glutamine (Q) residue, so called SQ or TQ consensus sites.
- ATM antibody
- ATR antibody
- DNA PK antibody
All lanes : Anti-Phospho SQ/TQ ATM/ATR Substrate antibody (ab130947) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Hela Whole Cell Lysate - UV Irradiated
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 469 kDa
Observed band size: 450 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes
This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab130947 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab130947 has been referenced in 2 publications.
- Cao L et al. Genotoxic stress-triggered ß-catenin/JDP2/PRMT5 complex facilitates reestablishing glutathione homeostasis. Nat Commun 10:3761 (2019). PubMed: 31434880
- Wang D et al. Colonic Lysine Homocysteinylation Induced by High-Fat Diet Suppresses DNA Damage Repair. Cell Rep 25:398-412.e6 (2018). PubMed: 30304680