Overview

  • Product name

    Phosphoenolpyruvic acid (PEP) Assay Kit
  • Detection method

    Colorimetric/Fluorometric
  • Sample type

    Urine, Serum, Plasma, Other biological fluids, Cell Lysate, Tissue Lysate
  • Assay type

    Enzyme activity
  • Sensitivity

    1 µM
  • Product overview

    Phosphoenolpyruvic acid (PEP) Assay Kit (ab204713) provides a convenient colorimetric and fluorometric means to measure PEP levels in various samples. In the assay, PEP is converted to ATP and pyruvate. The generated pyruvate is quantified by colorimetric (λmax = 570 nm) or fluorometric methods (Ex/Em = 535/587 nm). The assay is simple, sensitive and reliable. The detection limit is approximately 1 µM PEP in biological samples.


     

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    PEP Assay Buffer 1 x 25ml
    PEP Converter 1 vial
    PEP Developer Mix 1 vial
    PEP Probe (in DMSO) 1 x 0.2ml
    PEP Standard (1 µmole) 1 vial
  • Relevance

    Phosphoenolpyruvate (PEP) is an important intermediate in carbohydrate metabolism. Containing a high-energy phosphate bond, PEP is involved in glycolysis and gluco-neogenesis and in the shikimate pathway as well as in carbon fixation in plants. Bacteria utilize PEP in the phosphor-transferase system to acquire sugars from the environment. In the glycolytic pathway, PEP is formed from 2-phosphoglycerate by enolase and generates ATP through the action of pyruvate kinase.

Images

  • Typical PEP Standard calibration curve using colorimetric reading.

  • Typical PEP Standard calibration curve using fluorometric reading.

Protocols

References

ab204713 has not yet been referenced specifically in any publications.

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