Overview

  • Product name

    Phosphoglucomutase Activity Assay Kit (Fluorometric)
  • Detection method

    Fluorescent
  • Sample type

    Cell Lysate, Tissue Lysate
  • Assay type

    Enzyme activity (quantitative)
  • Sensitivity

    20 µU
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    Phosphoglucomutase Activity Assay Kit (Fluorometric) (ab211104) provides a sensitive, simple and convenient method for measuring Phosphoglucomutase (PGM) activity in plasma, cell and tissue lysates. The assay is based on the ability of Phosphoglucomutase to convert glucose-1-phosphate to glucose-6-phosphate. Glucose-6-phosphate is then oxidized by Glucose-6-phosphate dehydrogenase (G6PDH) to form NADPH, which in turn reduces the probe to generate an intense fluorescence product that can detected at Ex/Em = 535/587 nm.


    This assay can detect as low as 20 µU of Phosphoglucomutase activity.

  • Notes

    Phosphoglucomutase (PGM, EC 5.4.2.2) facilitates the interconversion of glucose-1-phosphate (G1P) and glucose-6-phosphate (G6P). Glycogen breakdown generates G1P, which is converted to G6P by PGM. G6P can then go into the glycolytic pathway to generate ATP, or to the pentose phosphate pathway to generate ribose and NADPH. On the other hand, when cells have extra energy, G6P is converted by PGM into G1P to generate glycogen.

    In humans, two isoforms have been described, PGM I and PGM II. PGM deficiency leads to glucose storage disease.

  • Platform

    Microplate reader

Properties

Images

  • PGM Activity Assay Kit (ab211104) Typical NADPH standard calibration curve.

  • PGM Activity Assay Kit (ab211104) Kinetic curves showing PGM activity in positive control (PosCtrl, included in kit), Jurkat cell lysate (0.4 μg) and rat liver homogenate (0.2 μg). Curves of background control wells are also shown.

  • PGM Activity Assay Kit (ab211104) Typical Phosphoglucomutase specific activity in Jurkat cell lysate (0.4 µg) and rat liver homogenate (0.2 µg).

Protocols

References

ab211104 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab211104.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up