Phosphoglucose Isomerase Activity Assay Kit (Colorimetric) (ab155897)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Adherent cells, Suspension cells, Tissue, Tissue Homogenate
- Sensitivity: 0.1 mU/well
Overview
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Product name
Phosphoglucose Isomerase Activity Assay Kit (Colorimetric)
See all Glucose 6 Phosphate Isomerase kits -
Detection method
Colorimetric -
Sample type
Tissue, Adherent cells, Suspension cells, Tissue Homogenate -
Assay type
Enzyme activity -
Sensitivity
< 0.1 mU/well -
Range
2.5 nmol/well - 12.5 nmol/well -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Abcam’s Phosphoglucose Isomerase Activity Assay Kit (Colorimetric) (ab155897) is an assay where PGI (phosphoglucose isomerase) converts fructose-6-phosphate to glucose-6-phosphate; the glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form a product, which reacts with a colorless probe to give strong absorbance at 450 nm. The PGI assay is simple, sensitive and rapid and can detect phosphoglucose isomerase activity less than 0.1 mU/reaction.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K775 Phosphoglucose Isomerase Activity Colorimetric Assay Kit. K775-100 is the same size as the 100 test size of ab155897.
Phosphoglucose isomerase (glucose-6-phosphate isomerase or PGI, EC 5.3.1.9) is an important housekeeping enzyme. PGI catalyzes the interconversion of glucose-6- phosphate to fructose-6-phosphate. PGI performs multiple functions & intracellularly plays key role in both glycolysis and gluconeogenesis. Extracellularly, PGI [also called Autocrine Motility Factor (AMF)] functions as a cytokine, which stimulates cell motility and is associated with tumor development and metastasis. In humans, PGI deficiency causes hemolytic anemia, whereas increased PGI activity is observed in many cancers such as gastrointestinal, kidney and breast cancer. Early detection of abnormal phosphoglucose isomerase activity is crucial for diagnosis, prediction and therapeutic strategy.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Assay Buffer V 1 x 27ml Developer Solution III 1 vial Development Enzyme Mix IX 1 vial NADH Standard I 1 vial Phosphoglucose Isomerase Positive Control 1 vial Phosphoglucose Isomerase Substrate 1 vial -
Research areas
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Relevance
This gene belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. -
Cellular localization
Cytoplasmic and Secreted -
Alternative names
- AMF
- Autocrine motility factor
- Glucose phosphate isomerase
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab155897 has been referenced in 2 publications.
- Sandberg TE et al. Synthetic cross-phyla gene replacement and evolutionary assimilation of major enzymes. Nat Ecol Evol 4:1402-1409 (2020). PubMed: 32778753
- Geng H et al. Interplay between hypoxia and androgen controls a metabolic switch conferring resistance to androgen/AR-targeted therapy. Nat Commun 9:4972 (2018). PubMed: 30478344