Overview

  • Product name
    Phosphoglucose Isomerase Activity Assay Kit (Colorimetric)
  • Sample type
    Tissue, Adherent cells, Suspension cells, Tissue Homogenate
  • Assay type
    Enzyme activity
  • Sensitivity
    < 0.1 mU/well
  • Range
    2.5 nmol/well - 12.5 nmol/well
  • Species reactivity
    Reacts with: Mammals, Other species
  • Product overview

    Abcam’s Phosphoglucose Isomerase Activity Assay Kit (Colorimetric) (ab155897) is an assay where PGI (phosphoglucose isomerase) converts fructose-6-phosphate to glucose-6-phosphate; the glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form a product, which reacts with a colorless probe to give strong absorbance at 450 nm. The PGI assay is simple, sensitive and rapid and can detect phosphoglucose isomerase activity less than 0.1 mU/reaction.

  • Notes

    Phosphoglucose isomerase (glucose-6-phosphate isomerase or PGI, EC 5.3.1.9) is an important housekeeping enzyme. PGI catalyzes the interconversion of glucose-6- phosphate to fructose-6-phosphate. PGI performs multiple functions & intracellularly plays key role in both glycolysis and gluconeogenesis. Extracellularly, PGI [also called Autocrine Motility Factor (AMF)] functions as a cytokine, which stimulates cell motility and is associated with tumor development and metastasis. In humans, PGI deficiency causes hemolytic anemia, whereas increased PGI activity is observed in many cancers such as gastrointestinal, kidney and breast cancer. Early detection of abnormal phosphoglucose isomerase activity is crucial for diagnosis, prediction and therapeutic strategy.

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    NADH Standard Yellow 1 vial
    PGI Assay Buffer WM 1 x 27ml
    PGI Developer Red 1 vial
    PGI Enzyme Mix Green 1 vial
    PGI Positive Control Purple 1 vial
    PGI Substrate Blue 1 vial
  • Research areas

Images

  • Phosphoglucose Isomerase activity in bovine liver, HeLa cell lysate and positive control

Protocols

References

ab155897 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Following ratios were analysed.
Protein X 1:5 Phosphoglucose Isomerase enzyme
Protein X 1:4 Phosphoglucose Isomerase enzyme
Protein X 1:3 Phosphoglucose Isomerase enzyme
Protein X 1:2 Phosphoglucose Isomerase enzyme
Protein X 1:1 Phosphoglucose Isomerase enzyme
Protein X 2:1 Phosphoglucose Isomerase enzyme
Protein X 3:1 Phosphoglucose Isomerase enzyme
Protein X 4:1 Phosphoglucose Isomerase enzyme
Protein X 5:1 Phosphoglucose Isomerase enzyme
Phosphoglucose isomerase activity
1:5 -1,26859
1:4 -2,05864
1:3 -1,79833
1:2 -2,39248
1:1 -1,2912
2:1 -2,80196
3:1 -1,72374
4:1 -2,97389
5:1 -2,36312
Our results indicate a downward trend (inhibitory effect) to be present of our protein towards the PGI activity. However, as some kind of parallel lines popped up from the data, we would consider the data inconclusive as such. A re-analysis would be necessary.
But, as the positive control can only be reconstituted with 20 µl of assay buffer, we think the kit is not suitable for activation-inhibition experiments at the moment.

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Verified customer

Submitted Sep 11 2018

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