Overview

  • Product name

    Anti-Phospholamban antibody
    See all Phospholamban primary antibodies
  • Description

    Rabbit polyclonal to Phospholamban
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Rabbit, Cow, Dog, Human, Pig
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-11 of Human Phospholamban (UniProt P26678).

  • Positive control

    • Mouse heart whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab126174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 6 kDa.

Target

  • Function

    Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation.
  • Tissue specificity

    Heart muscle (at protein level).
  • Involvement in disease

    Cardiomyopathy, dilated 1P
    Cardiomyopathy, familial hypertrophic 18
  • Sequence similarities

    Belongs to the phospholamban family.
  • Post-translational
    modifications

    Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes.
  • Cellular localization

    Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. Mitochondrion membrane. Membrane. Colocalizes with HAX1 at the endoplasmic reticulum (PubMed:17241641). Colocalizes with DMPK a the sarcoplasmic reticulum (PubMed:15598648).
  • Information by UniProt
  • Database links

  • Alternative names

    • Cardiac phospholamban antibody
    • CMD1P antibody
    • CMH18 antibody
    • PLB antibody
    • Pln antibody
    • PPLA_HUMAN antibody
    see all

Images

  • Anti-Phospholamban antibody (ab126174) at 1/5000 dilution + Mouse heart whole cell lysate at 50 µg

    Predicted band size: 6 kDa



    15% SDS PAGE

References

This product has been referenced in:

  • Quan C  et al. SPEG Controls Calcium Re-Uptake into the Sarcoplasmic Reticulum Through Regulating SERCA2a by Its Second Kinase-Domain. Circ Res N/A:N/A (2018). Read more (PubMed: 30566039) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (heart)
Permeabilization
Yes - NP-40
Specification
heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Fixative
Paraformaldehyde

Francesca Stillitano

Verified customer

Submitted Nov 16 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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