Question (56717) | Anti-Phospholamban antibody (ab85146)

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Question

Hi: I want to detect the protein expression of Phospholamban and Phospholamban(phospho S16) on Guinea pig bladder smoothe muscle, in the presence or absence of drugs by western blot.So I want to know as following about ab86930, ab85146 and ab15000 as following: 1. What is the difference between ab86930 and ab85146? Which is more suitable for guinea pig? 2. Are there other antibodies more suitable for Phospholamban and Phospholamban(phospho S16) on guinea pig? 3.Which lysate solution is best for Phospholamban and Phospholamban(phospho S16)? 4.Which protease and phosphatase inhibitors are best for the two protein? 5.Would you like give the protocal for the phosphorylation protein,such as phospho S16?

Answer

Thank you for contacting Abcam.

I would be very happy to help answer your questions:

1 - What is the difference between ab86930 and ab85146? Which is more suitable for guinea pig?

- Both antibodies target different areas of the Phospholamban protein. For a lot of targets, Abcam offers multiply antibodies, that either target different domains, or are more suited to different applications, depending on the customers’ needs.

I cannot find the guinea pig sequence for Phospholamban, therefore I cannot make a recommendation as to whether either of these antibodies would work for that species. If you could please supply the guinea pig sequence, I would be happy to do the alignment and see if there is a chance that either will work for you.

2 - Are there other antibodies more suitable for Phospholamban and Phospholamban(phospho S16) on guinea pig?

- Again, without the guinea pig sequence, I cannot confidently answer this question.

3 - Which lysate solution is best for Phospholamban and Phospholamban(phospho S16)?

- From the information available on the webpages, it looks as though mouse, rat, human and bovine cardiac tissue either treated with or without PKA would be suitable lysates.

4 - Which protease and phosphatase inhibitors are best for the two protein?

- This would be determined on your exact experiment at hand, below is a link to some protease inhibitors, however if you require phospahatse inhibitors then, we unfortunately do not carry those in our catalogue:

https://www.abcam.com/Protease-Inhibitor-Cocktail-ab65621.html



5. Would you like give the protocol for the phosphorylation protein,such as phospho S16?

- For ab15000 was treated with PKA to induce phosphorylation.

Please let me know if there is anything else I can help you with, or if you would like to me to perform the alignment between the guinea pig sequence and the immunogen sequences for these antibodies.

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