Name: Anti-Phospholamban antibody [EPR21897]
Brand: RabMAb
SKU: ab219626

Product name

Anti-Phospholamban antibody [EPR21897]
See all Phospholamban primary antibodies
Description

Rabbit monoclonal [EPR21897] to Phospholamban
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, IPmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment within Human Phospholamban aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P26678

Run BLAST with Run BLAST with
Positive control
- WB: Human heart and fetal heart tissue lysates; Mouse heart tissue lysate; Rat heart tissue lysate. IHC-P: Human, mouse and rat cardiac muscle tissue. IP: Human fetal heart tissue lysate.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21897
Isotype

IgG
Research areas

Alternative Versions
- Anti-Phospholamban antibody [EPR21897] - BSA and Azide free (ab234903)
Compatible Secondaries
- VeriBlot for IP Detection Reagent (HRP) (ab131366)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Related Products
- Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

Our Abpromise guarantee covers the use of ab219626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/2000. Detects a band of approximately 10, 25 kDa (predicted molecular weight: 6 kDa).
IHC-P		1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP		1/30.

Function

Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation.
Tissue specificity

Heart muscle (at protein level).
Involvement in disease

Cardiomyopathy, dilated 1P
Cardiomyopathy, familial hypertrophic 18
Sequence similarities

Belongs to the phospholamban family.
Post-translational
modifications

Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes.
Cellular localization

Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. Mitochondrion membrane. Membrane. Colocalizes with HAX1 at the endoplasmic reticulum (PubMed:17241641). Colocalizes with DMPK a the sarcoplasmic reticulum (PubMed:15598648).
Target information above from: UniProt accession P26678 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 5350 Human
- Entrez Gene: 18821 Mouse
- Entrez Gene: 64672 Rat
- Omim: 172405 Human
- SwissProt: P26678 Human
- SwissProt: P61014 Mouse
- SwissProt: P61016 Rat
- Unigene: 170839 Human
- Unigene: 745010 Human
- Unigene: 34145 Mouse
- Unigene: 9740 Rat
see all
Alternative names
- Cardiac phospholamban antibody
- CMD1P antibody
- CMH18 antibody
- PLB antibody
- Pln antibody
- PPLA_HUMAN antibody
see all

Western blot - Anti-Phospholamban antibody [EPR21897] (ab219626)

All lanes : Anti-Phospholamban antibody [EPR21897] (ab219626) at 1/2000 dilution

Lane 1 : Human heart tissue lysate
Lane 2 : Human fetal heart tissue lysate
Lane 3 : Mouse heart tissue lysate
Lanes 4-5 : Rat heart tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 6 kDa
Observed band size: 10,25 kDa
why is the actual band size different from the predicted?

Exposure time: 15 seconds

Blocking and dilution buffer: 5% NFDM/TBST.

WB bands are consistent with reference (PMID 20696159; PMID 22144574)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholamban antibody [EPR21897] (ab219626)

Immunohistochemical analysis of paraffin embedded rat cardiac muscle tissue labeling Phospholamban with ab219626 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human cardiac muscle (PMID: 28759816, PMID: 27642167) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholamban antibody [EPR21897] (ab219626)

Negative control: No staining shown in human tonsil tissue.

Immunohistochemical analysis of paraffin embedded human tonsil tissue labeling Phospholamban with ab219626 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. No staining on human tonsil is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholamban antibody [EPR21897] (ab219626)

Immunohistochemical analysis of paraffin embedded mouse cardiac muscle tissue labeling Phospholamban with ab219626 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on mouse cardiac muscle (PMID: 28759816, PMID: 27642167) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunoprecipitation - Anti-Phospholamban antibody [EPR21897] (ab219626)

Phospholamban was immunoprecipitated from 0.35 mg of human fetal heart tissue lysate with ab219626 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab219626 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

Lane 1: Human fetal heart tissue lysate 10 μg (Input).
Lane 2: ab219626 IP in human fetal heart tissue lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab219626 in human fetal heart tissue lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholamban antibody [EPR21897] (ab219626)

Immunohistochemical analysis of paraffin embedded human cardiac muscle tissue labeling Phospholamban with ab219626 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human cardiac muscle (PMID: 28759816, PMID: 27642167) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Datasheet

ab219626 has not yet been referenced specifically in any publications.

Publishing research using ab219626? Please let us know so that we can cite the reference in this datasheet.

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Anti-Phospholamban antibody [EPR21897] (ab219626)

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