Recombinant Anti-Phospholamban antibody [EPR21897] (ab219626)
![Western blot - Anti-Phospholamban antibody [EPR21897] (ab219626)](https://www.abcam.com/ps/products/219/ab219626/Images/ab219626-309251-anti-phospholamban-antibody-epr21897-western-blot.jpg "Western blot - Anti-Phospholamban antibody [EPR21897] (ab219626)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21897] to Phospholamban
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Phospholamban antibody [EPR21897]
See all Phospholamban primary antibodies -
Description
Rabbit monoclonal [EPR21897] to Phospholamban -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human heart and fetal heart tissue lysates; Mouse heart tissue lysate; Rat heart tissue lysate. IHC-P: Human, mouse and rat cardiac muscle tissue. IP: Human fetal heart tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21897 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab219626 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000. Detects a band of approximately 10, 25 kDa (predicted molecular weight: 6 kDa).
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| IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| IP |
1/30.
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| Notes |
|---|
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WB
1/2000. Detects a band of approximately 10, 25 kDa (predicted molecular weight: 6 kDa). |
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IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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IP
1/30. |
Target
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Function
Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation. -
Tissue specificity
Heart muscle (at protein level). -
Involvement in disease
Cardiomyopathy, dilated 1P
Cardiomyopathy, familial hypertrophic 18 -
Sequence similarities
Belongs to the phospholamban family. -
Post-translational
modificationsPhosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes. -
Cellular localization
Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. Mitochondrion membrane. Membrane. Colocalizes with HAX1 at the endoplasmic reticulum (PubMed:17241641). Colocalizes with DMPK a the sarcoplasmic reticulum (PubMed:15598648). - Information by UniProt
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Database links
- Entrez Gene: 5350 Human
- Entrez Gene: 18821 Mouse
- Entrez Gene: 64672 Rat
- Omim: 172405 Human
- SwissProt: P26678 Human
- SwissProt: P61014 Mouse
- SwissProt: P61016 Rat
- Unigene: 170839 Human
see all -
Alternative names
- Cardiac phospholamban antibody
- CMD1P antibody
- CMH18 antibody
see all
Images
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Western blot - Anti-Phospholamban antibody [EPR21897] (ab219626)All lanes : Anti-Phospholamban antibody [EPR21897] (ab219626) at 1/2000 dilution
Lane 1 : Human heart tissue lysate
Lane 2 : Human fetal heart tissue lysate
Lane 3 : Mouse heart tissue lysate
Lanes 4-5 : Rat heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 6 kDa
Observed band size: 10,25 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking and dilution buffer: 5% NFDM/TBST.
WB bands are consistent with reference (PMID 20696159; PMID 22144574)
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholamban antibody [EPR21897] (ab219626)](https://www.abcam.com/ps/products/219/ab219626/Images/ab219626-309249-anti-phospholamban-antibody-epr21897-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholamban antibody [EPR21897] (ab219626)Immunohistochemical analysis of paraffin embedded rat cardiac muscle tissue labeling Phospholamban with ab219626 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human cardiac muscle (PMID: 28759816, PMID: 27642167) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholamban antibody [EPR21897] (ab219626)](https://www.abcam.com/ps/products/219/ab219626/Images/ab219626-309250-anti-phospholamban-antibody-epr21897-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholamban antibody [EPR21897] (ab219626)Negative control: No staining shown in human tonsil tissue.
Immunohistochemical analysis of paraffin embedded human tonsil tissue labeling Phospholamban with ab219626 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. No staining on human tonsil is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholamban antibody [EPR21897] (ab219626)](https://www.abcam.com/ps/products/219/ab219626/Images/ab219626-309248-anti-phospholamban-antibody-epr21897-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholamban antibody [EPR21897] (ab219626)Immunohistochemical analysis of paraffin embedded mouse cardiac muscle tissue labeling Phospholamban with ab219626 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on mouse cardiac muscle (PMID: 28759816, PMID: 27642167) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Immunoprecipitation - Anti-Phospholamban antibody [EPR21897] (ab219626)](https://www.abcam.com/ps/products/219/ab219626/Images/ab219626-309252-anti-phospholamban-antibody-epr21897-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Phospholamban antibody [EPR21897] (ab219626)Phospholamban was immunoprecipitated from 0.35 mg of human fetal heart tissue lysate with ab219626 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab219626 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Human fetal heart tissue lysate 10 μg (Input).
Lane 2: ab219626 IP in human fetal heart tissue lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab219626 in human fetal heart tissue lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholamban antibody [EPR21897] (ab219626)](https://www.abcam.com/ps/products/219/ab219626/Images/ab219626-309247-anti-phospholamban-antibody-epr21897-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholamban antibody [EPR21897] (ab219626)Immunohistochemical analysis of paraffin embedded human cardiac muscle tissue labeling Phospholamban with ab219626 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human cardiac muscle (PMID: 28759816, PMID: 27642167) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Anti-Phospholamban antibody [EPR21897] (ab219626)](https://www.abcam.com/ps/products/219/ab219626/Images/ab219626-7-benefits-of-recombinant-antibodies.png)
Anti-Phospholamban antibody [EPR21897] (ab219626)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab219626 has not yet been referenced specifically in any publications.