Anti-Phospholamban (phospho S16) antibody (ab15000)
Key features and details
- Rabbit polyclonal to Phospholamban (phospho S16)
- Suitable for: IHC-Fr, IHC-P, WB
- Reacts with: Mouse, Rat, Rabbit, Hamster, Cow, Human, Pig, Chinese hamster
- Isotype: IgG
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Overview
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Product name
Anti-Phospholamban (phospho S16) antibody
See all Phospholamban primary antibodies -
Description
Rabbit polyclonal to Phospholamban (phospho S16) -
Host species
Rabbit -
Specificity
Multiple bands can be observed in addition to the 25kD pentamer for PLB. Monomeric, dimeric, trimeric forms, etc. can be observed if the preparations are boiled before SDS-PAGE. This antibody also can display non-specific bands at 75 and 100kD in some lysate preparations. Note: Do not boil cardiac preparation. Boiling may reduce the 25kD pentamer in to tri-, di- or monomers. In addition, phosphorylation may disrupt the pentamer into dimers and monomers. -
Tested applications
Suitable for: IHC-Fr, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Rabbit, Hamster, Cow, Human, Pig, Chinese hamster
Predicted to work with: Dog -
Immunogen
Synthetic peptide corresponding to Human Phospholamban aa 1-100 (phospho S16) conjugated to keyhole limpet haemocyanin.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.368% Tris glycine, 30% Glycerol, 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab15000 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr | (1) |
Use at an assay dependent concentration.
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IHC-P | (1) |
Use at an assay dependent concentration.
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WB | (8) |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 6.24 kDa.
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Notes |
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IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 6.24 kDa. |
Target
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Function
Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation. -
Tissue specificity
Heart muscle (at protein level). -
Involvement in disease
Cardiomyopathy, dilated 1P
Cardiomyopathy, familial hypertrophic 18 -
Sequence similarities
Belongs to the phospholamban family. -
Post-translational
modificationsPhosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes. -
Cellular localization
Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. Mitochondrion membrane. Membrane. Colocalizes with HAX1 at the endoplasmic reticulum (PubMed:17241641). Colocalizes with DMPK a the sarcoplasmic reticulum (PubMed:15598648). - Information by UniProt
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Database links
- Entrez Gene: 5350 Human
- Entrez Gene: 18821 Mouse
- Entrez Gene: 64672 Rat
- Omim: 172405 Human
- SwissProt: A4IFH6 Cow
- SwissProt: P61012 Dog
- SwissProt: P26678 Human
- SwissProt: P61014 Mouse
see all -
Alternative names
- Cardiac phospholamban antibody
- CMD1P antibody
- CMH18 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (33)
ab15000 has been referenced in 33 publications.
- Benkner A et al. Riociguat attenuates the changes in left ventricular proteome and microRNA profile after experimental aortic stenosis in mice. Br J Pharmacol 179:4575-4592 (2022). PubMed: 35751875
- Li H et al. Notoginsenoside R1-loaded mesoporous silica nanoparticles targeting the site of injury through inflammatory cells improves heart repair after myocardial infarction. Redox Biol 54:102384 (2022). PubMed: 35777198
- Wang W et al. RETRACTED ARTICLE: Inhibition of miR-296-5p protects the heart from cardiac hypertrophy by targeting CACNG6. Gene Ther 28:391 (2021). PubMed: 31844153
- Xu D et al. Xanthine oxidase inhibitor febuxostat reduces atrial fibrillation susceptibility by inhibition of oxidized CaMKII in Dahl salt-sensitive rats. Clin Sci (Lond) 135:2409-2422 (2021). PubMed: 34386810
- Zheng J et al. Stachydrine hydrochloride suppresses phenylephrine-induced pathological cardiac hypertrophy by inhibiting the calcineurin/nuclear factor of activated T-cell signalling pathway. Eur J Pharmacol 883:173386 (2020). PubMed: 32712088