Overview

  • Product name

    Anti-Phospholamban (phospho S16) antibody [EPR1911]
    See all Phospholamban primary antibodies
  • Description

    Rabbit monoclonal [EPR1911] to Phospholamban (phospho S16)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding Serine 16 of Human Phospholamban.

  • Positive control

    • Fetal heart lysate
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab92697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: 1/50.
    WB: 1/200,000 - 1/500,000. Detects a band of approximately 11 kDa (predicted molecular weight: 6 kDa).

    Is unsuitable for IHC-P.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation.
    • Tissue specificity

      Heart muscle (at protein level).
    • Involvement in disease

      Cardiomyopathy, dilated 1P
      Cardiomyopathy, familial hypertrophic 18
    • Sequence similarities

      Belongs to the phospholamban family.
    • Post-translational
      modifications

      Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes.
    • Cellular localization

      Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. Mitochondrion membrane. Membrane. Colocalizes with HAX1 at the endoplasmic reticulum (PubMed:17241641). Colocalizes with DMPK a the sarcoplasmic reticulum (PubMed:15598648).
    • Information by UniProt
    • Database links

    • Alternative names

      • Cardiac phospholamban antibody
      • CMD1P antibody
      • CMH18 antibody
      • PLB antibody
      • Pln antibody
      • PPLA_HUMAN antibody
      see all

    Images

    • All lanes : Anti-Phospholamban (phospho S16) antibody [EPR1911] (ab92697) at 1/200000 dilution

      Lane 1 : Fetal heart lysate (untreated)
      Lane 2 : Fetal heart lysate (treated with LP)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit antibody at 1/2000 dilution

      Predicted band size: 6 kDa



      Actual band size is 11 KDa.

    References

    This product has been referenced in:

    • Molina CE  et al. Profibrotic, Electrical, and Calcium-Handling Remodeling of the Atria in Heart Failure Patients With and Without Atrial Fibrillation. Front Physiol 9:1383 (2018). Read more (PubMed: 30356673) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab92697.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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