Product nameAnti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481]
See all Phospholipase A2 activator protein (PLAP) primary antibodies
DescriptionRabbit monoclonal [EPR5481] to Phospholipase A2 activator protein (PLAP)
Tested applicationsSuitable for: WB, IHC-P, IP, ICC/IFmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Human
Synthetic peptide within Human Phospholipase A2 activator protein (PLAP) aa 700-800 (internal sequence). The exact sequence is proprietary.
- MCF7, K562 and HepG2 whole cell lysate (ab7900); Human kidney tissue
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.24 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133589 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 87 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||1/10 - 1/100.|
|ICC/IF||1/50 - 1/100.|
FunctionInvolved in the maintenance of ubiquitin levels.
Sequence similaritiesBelongs to the WD repeat PLAP family.
Contains 6 ARM repeats.
Contains 1 PFU domain.
Contains 1 PUL domain.
Contains 7 WD repeats.
DomainThe PUL domain is composed of 6 armadillo-like repeats and mediates the interaction with VCP C-terminus.
The PFU domain mediates interaction with ubiquitin.
- Information by UniProt
- FLJ11281 antibody
- FLJ12699 antibody
- Glycerophosphatase antibody
All lanes : Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 87 kDa
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Phospholipase A2 activator protein (PLAP) with ab133589 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab133589 has not yet been referenced specifically in any publications.