Recombinant
RabMAb

Recombinant Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589)

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Overview

  • Product name

    Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481]
    See all Phospholipase A2 activator protein (PLAP) primary antibodies

  • Description

    Rabbit monoclonal [EPR5481] to Phospholipase A2 activator protein (PLAP)

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Phospholipase A2 activator protein (PLAP) aa 700-800 (internal sequence). The exact sequence is proprietary.

  • Positive control

    • MCF7, K562 and HepG2 whole cell lysate (ab7900); Human kidney tissue

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 1.24 x 10 -10 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR5481

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 87 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/10 - 1/100.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function

      Involved in the maintenance of ubiquitin levels.

    • Sequence similarities

      Belongs to the WD repeat PLAP family.
      Contains 6 ARM repeats.
      Contains 1 PFU domain.
      Contains 1 PUL domain.
      Contains 7 WD repeats.

    • Domain

      The PUL domain is composed of 6 armadillo-like repeats and mediates the interaction with VCP C-terminus.
      The PFU domain mediates interaction with ubiquitin.
    • Information by UniProt

    • Database links

      • Alternative names

        • FLJ11281 antibody
        • FLJ12699 antibody
        • Glycerophosphatase antibody
        • OTTHUMP00000045176 antibody
        • Phospholipase A 2 activating protein antibody
        • Phospholipase A-2-activating protein antibody
        • Phospholipase A2 activating protein antibody
        • PLA2P antibody
        • Plaa antibody
        • PLAP antibody
        • PLAP_HUMAN antibody
        see all

      Images

      • Western blot - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589)
        Western blot - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589)
        All lanes : Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589) at 1/1000 dilution

        Lane 1 : MCF7 cell lysate
        Lane 2 : K562 cell lysate
        Lane 3 : HepG2 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

        Predicted band size: 87 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589)

        Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Phospholipase A2 activator protein (PLAP) with ab133589 at 1/50 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Other - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589)
        Other - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

      References

      ab133589 has not yet been referenced specifically in any publications.

      Publishing research using ab133589? Please let us know so that we can cite the reference in this datasheet.

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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