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Immunology Innate Immunity Macrophage / Inflamm.
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589)

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Western blot - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589)
  • Western blot - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589)
  • OI-RD Scanning - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589)
  • Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5481] to Phospholipase A2 activator protein (PLAP)
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

You may also be interested in

Knockout
Product image
Human PLAA knockout HeLa cell line (ab265255)

View more associated products

Overview

  • Product name

    Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481]
    See all Phospholipase A2 activator protein (PLAP) primary antibodies
  • Description

    Rabbit monoclonal [EPR5481] to Phospholipase A2 activator protein (PLAP)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Phospholipase A2 activator protein (PLAP) aa 700-800 (internal sequence). The exact sequence is proprietary.

  • Positive control

    • WB: HeLa, MCF7, K562 and HepG2 whole cell lysate (ab7900). IHC-P: Human kidney tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 1.24 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR5481
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.

Associated products

  • Alternative Versions

    • Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] - BSA and Azide free (ab248568)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human PLAA knockout HeLa cell line (ab265255)
  • KO cell lysates

    • Human PLAA knockout HeLa cell lysate (ab258121)
  • Positive Controls

    • K562 whole cell lysate (ab7911)

Applications

Our Abpromise guarantee covers the use of ab133589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 87 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Involved in the maintenance of ubiquitin levels.
    • Sequence similarities

      Belongs to the WD repeat PLAP family.
      Contains 6 ARM repeats.
      Contains 1 PFU domain.
      Contains 1 PUL domain.
      Contains 7 WD repeats.
    • Domain

      The PUL domain is composed of 6 armadillo-like repeats and mediates the interaction with VCP C-terminus.
      The PFU domain mediates interaction with ubiquitin.
    • Target information above from: UniProt accession Q9Y263 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 9373 Human
      • Omim: 603873 Human
      • SwissProt: Q9Y263 Human
      • Unigene: 27182 Human
      • Alternative names

        • FLJ11281 antibody
        • FLJ12699 antibody
        • Glycerophosphatase antibody
        • OTTHUMP00000045176 antibody
        • Phospholipase A 2 activating protein antibody
        • Phospholipase A-2-activating protein antibody
        • Phospholipase A2 activating protein antibody
        • PLA2P antibody
        • Plaa antibody
        • PLAP antibody
        • PLAP_HUMAN antibody
        see all

      Images

      • Western blot - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589)
        Western blot - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589)
        All lanes : Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589) at 1/1000 dilution

        Lane 1 : Wild-type HeLa cell lysate
        Lane 2 : PLAP knockout HeLa cell lysate
        Lane 3 : MCF7 cell lysate
        Lane 4 : Daudi cell lysate

        Lysates/proteins at 20 µg per lane.

        Performed under reducing conditions.

        Predicted band size: 87 kDa



        Lanes 1-4: Merged signal (red and green). Green - ab133589 observed at 87 kDa. Red - loading control ab8245 observed at 37 kDa.

         ab133589 Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] was shown to specifically react with PLAP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265255 (knockout cell lysate ab258121) was used. Wild-type and PLAP knockout samples were subjected to SDS-PAGE. ab133589 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

         

      • Western blot - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589)
        Western blot - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589)
        All lanes : Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589) at 1/1000 dilution

        Lane 1 : MCF7 cell lysate
        Lane 2 : K562 cell lysate
        Lane 3 : HepG2 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

        Predicted band size: 87 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589)

        Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Phospholipase A2 activator protein (PLAP) with ab133589 at 1/50 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • OI-RD Scanning - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589)
        OI-RD Scanning - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD
      • Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589)
        Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589)

      Protocols

      • Immunohistochemistry protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab133589? Please let us know so that we can cite the reference in this datasheet.

      ab133589 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
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      There are currently no Customer reviews or Questions for ab133589.
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