Recombinant Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589)
![Western blot - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589)](https://www.abcam.com/ps/products/133/ab133589/Images/ab133589-389720-anti-phospholipase-a2-activator-protein-plap-antibody-epr5481-western-blot-human-lysate.jpg "Western blot - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5481] to Phospholipase A2 activator protein (PLAP)
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481]
See all Phospholipase A2 activator protein (PLAP) primary antibodies -
Description
Rabbit monoclonal [EPR5481] to Phospholipase A2 activator protein (PLAP) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Phospholipase A2 activator protein (PLAP) aa 700-800 (internal sequence). The exact sequence is proprietary.
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Positive control
- WB: HeLa, MCF7, K562 and HepG2 whole cell lysate (ab7900). IHC-P: Human kidney tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.24 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5481 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab133589 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 87 kDa. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Involved in the maintenance of ubiquitin levels. -
Sequence similarities
Belongs to the WD repeat PLAP family.
Contains 6 ARM repeats.
Contains 1 PFU domain.
Contains 1 PUL domain.
Contains 7 WD repeats. -
Domain
The PUL domain is composed of 6 armadillo-like repeats and mediates the interaction with VCP C-terminus.
The PFU domain mediates interaction with ubiquitin. - Information by UniProt
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Database links
- Entrez Gene: 9373 Human
- Omim: 603873 Human
- SwissProt: Q9Y263 Human
- Unigene: 27182 Human
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Alternative names
- FLJ11281 antibody
- FLJ12699 antibody
- Glycerophosphatase antibody
see all
Images
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Western blot - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589)All lanes : Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PLAP knockout HeLa cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 87 kDaLanes 1-4: Merged signal (red and green). Green - ab133589 observed at 87 kDa. Red - loading control ab8245 observed at 37 kDa.
ab133589 Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] was shown to specifically react with PLAP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265255 (knockout cell lysate ab258121) was used. Wild-type and PLAP knockout samples were subjected to SDS-PAGE. ab133589 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589)](https://www.abcam.com/ps/products/133/ab133589/Images/ab133589-147403-anti-phospholipase-a2-activator-protein-plap-antibody-epr5481-western-blot.jpg)
Western blot - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589)All lanes : Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 87 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589)](https://www.abcam.com/ps/products/133/ab133589/Images/ab133589-147402-anti-phospholipase-a2-activator-protein-plap-antibody-epr5481-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Phospholipase A2 activator protein (PLAP) with ab133589 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589)](https://www.abcam.com/ps/products/133/ab133589/Images/ab133589-147401-anti-phospholipase-a2-activator-protein-plap-antibody-epr5481.jpg)
OI-RD Scanning - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589) -
![Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589)](https://www.abcam.com/ps/products/133/ab133589/Images/ab133589-5-benefits-of-recombinant-antibodies.png)
Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (ab133589)
Protocols
Datasheets and documents
References (0)
ab133589 has not yet been referenced specifically in any publications.
