Product nameAnti-Phospholipase A2 activator protein (PLAP) antibody [Y102]
See all Phospholipase A2 activator protein (PLAP) primary antibodies
DescriptionRabbit monoclonal [Y102] to Phospholipase A2 activator protein (PLAP)
Specificityab32354 recognises Phospholipase A2 activator protein (PLAP).
Tested applicationsSuitable for: WB, IHC-P, ICCmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Human
Synthetic peptide within Human Phospholipase A2 activator protein (PLAP) aa 1-100 (N terminal). The exact sequence is proprietary.
- MCF-7 and K562 cell lysates and human lung metastatic cancer tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab32354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 81 kDa (predicted molecular weight: 80 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionInvolved in the maintenance of ubiquitin levels.
Sequence similaritiesBelongs to the WD repeat PLAP family.
Contains 6 ARM repeats.
Contains 1 PFU domain.
Contains 1 PUL domain.
Contains 7 WD repeats.
DomainThe PUL domain is composed of 6 armadillo-like repeats and mediates the interaction with VCP C-terminus.
The PFU domain mediates interaction with ubiquitin.
- Information by UniProt
- FLJ11281 antibody
- FLJ12699 antibody
- Glycerophosphatase antibody
All lanes : Anti-Phospholipase A2 activator protein (PLAP) antibody [Y102] (ab32354) at 1/1000 dilution
Lane 1 : MCF-7 cell lysate
Lane 2 : K562 cell lysate
Predicted band size: 80 kDa
Observed band size: 81 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin-embedded human lung metastatic cancer using ab32354 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This product has been referenced in:
- Zhang F et al. Alteration in the activation state of new inflammation-associated targets by phospholipase A2-activating protein (PLAA). Cell Signal 20:844-61 (2008). Read more (PubMed: 18291623) »
- Alexandru G et al. UBXD7 binds multiple ubiquitin ligases and implicates p97 in HIF1alpha turnover. Cell 134:804-16 (2008). Read more (PubMed: 18775313) »