Recombinant
RabMAb

Recombinant Anti-Phospholipase A2 activator protein (PLAP) antibody [Y102] (ab32354)

Overview

  • Product name

    Anti-Phospholipase A2 activator protein (PLAP) antibody [Y102]
    See all Phospholipase A2 activator protein (PLAP) primary antibodies
  • Description

    Rabbit monoclonal [Y102] to Phospholipase A2 activator protein (PLAP)
  • Host species

    Rabbit
  • Specificity

    ab32354 recognises Phospholipase A2 activator protein (PLAP).
  • Tested applications

    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Phospholipase A2 activator protein (PLAP) aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • MCF-7 and K562 cell lysates and human lung metastatic cancer tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    IgG fraction
  • Clonality

    Monoclonal
  • Clone number

    Y102
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab32354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 81 kDa (predicted molecular weight: 80 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/50.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Involved in the maintenance of ubiquitin levels.
    • Sequence similarities

      Belongs to the WD repeat PLAP family.
      Contains 6 ARM repeats.
      Contains 1 PFU domain.
      Contains 1 PUL domain.
      Contains 7 WD repeats.
    • Domain

      The PUL domain is composed of 6 armadillo-like repeats and mediates the interaction with VCP C-terminus.
      The PFU domain mediates interaction with ubiquitin.
    • Information by UniProt
    • Database links

    • Alternative names

      • FLJ11281 antibody
      • FLJ12699 antibody
      • Glycerophosphatase antibody
      • OTTHUMP00000045176 antibody
      • Phospholipase A 2 activating protein antibody
      • Phospholipase A-2-activating protein antibody
      • Phospholipase A2 activating protein antibody
      • PLA2P antibody
      • Plaa antibody
      • PLAP antibody
      • PLAP_HUMAN antibody
      see all

    Images

    • All lanes : Anti-Phospholipase A2 activator protein (PLAP) antibody [Y102] (ab32354) at 1/1000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : K562 cell lysate

      Predicted band size: 80 kDa
      Observed band size: 81 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemical analysis of paraffin-embedded human lung metastatic cancer using ab32354 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Zhang F  et al. Alteration in the activation state of new inflammation-associated targets by phospholipase A2-activating protein (PLAA). Cell Signal 20:844-61 (2008). Read more (PubMed: 18291623) »
    • Alexandru G  et al. UBXD7 binds multiple ubiquitin ligases and implicates p97 in HIF1alpha turnover. Cell 134:804-16 (2008). Read more (PubMed: 18775313) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Answer

    Thank you for your reply. I would be happy to provide a replacement vial of ab18184. To do so I will just need to access the original order on our system. Please provide the Abcam order reference number or an internal purchase order number so I can process your request. If you have purchased the antibody through one of our distributors, please request the order information from them. I look forward to hearing from you. Please let me know if you have any other questions

    Read More
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (fibroblasts culture)
    Loading amount
    30 µg
    Specification
    fibroblasts culture
    Gel Running Conditions
    Reduced Denaturing (10% SDS-page gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Dr. Yishay Shoval

    Verified customer

    Submitted Jan 04 2012

    Question
    Answer

    Thank you for your enquiry. The exact location of the peptide is proprietary information but it is between aa 70 - 90. I hope this helps. Please do not hesitate to contact us if you have any more questions.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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