Anti-phospholipase A2 antibody (ab23705)
Key features and details
- Rabbit polyclonal to Phospholipase A2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-phospholipase A2 antibody
See all Phospholipase A2 primary antibodies -
Description
Rabbit polyclonal to Phospholipase A2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Does not react with: Rabbit, Cow, Pig -
Immunogen
Human recombinant sPLA2 (Type IIa).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab23705 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration.
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WB |
1/1000. Detects a band of approximately 14 kDa.
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Notes |
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IHC-P
Use at an assay dependent concentration. |
WB
1/1000. Detects a band of approximately 14 kDa. |
Target
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Function
Thought to participate in the regulation of the phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. -
Sequence similarities
Belongs to the phospholipase A2 family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 5320 Human
- Omim: 172411 Human
- SwissProt: P14555 Human
- Unigene: 466804 Human
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Alternative names
- EC 3.1.1.4 antibody
- GIIC sPLA2 antibody
- Group IIA phospholipase A2 antibody
see all
Images
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Western blot detection of human phospholipase A2 (25µg)
ab23705 at 1/1000 western blot detection of 0.25 ug of human phospolipase A2.
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IHC image of ab23705 staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab23705, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
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Datasheet download
References (16)
ab23705 has been referenced in 16 publications.
- Falidas E et al. Secretory Phospholipase A2 Digital Expression Analysis in Colon Adenocarcinoma. In Vivo 36:738-742 (2022). PubMed: 35241529
- Wang Y et al. Single-cell analysis of pancreatic ductal adenocarcinoma identifies a novel fibroblast subtype associated with poor prognosis but better immunotherapy response. Cell Discov 7:36 (2021). PubMed: 34035226
- Wei Y et al. Phospholipase A2 inhibitor-loaded micellar nanoparticles attenuate inflammation and mitigate osteoarthritis progression. Sci Adv 7:N/A (2021). PubMed: 33827816
- Mohammadtursun N et al. Loki zupa alleviates inflammatory and fibrotic responses in cigarette smoke induced rat model of chronic obstructive pulmonary disease. Chin Med 15:92 (2020). PubMed: 32874197
- Papadopoulos S et al. Secretory Phospholipase A2-IIA Protein and mRNA Pools in Extracellular Vesicles of Bronchoalveolar Lavage Fluid from Patients with Early Acute Respiratory Distress Syndrome: A New Perception in the Dissemination of Inflammation? Pharmaceuticals (Basel) 13:N/A (2020). PubMed: 33238426