Anti-Phospholipase A2 IID antibody (ab47118)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-Phospholipase A2 IID antibody
See all Phospholipase A2 IID primary antibodies -
DescriptionRabbit polyclonal to Phospholipase A2 IID
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Host speciesRabbit
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SpecificityThis antibody is specific for Phospholipase A2 IID.
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Tested applicationsSuitable for: ICC/IF, Functional Studies, ELISA, WBmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant human Phospholipase A2 IID. 16.4 kDa protein containing 125 amino acid residues of the human Phospholipase A2 IID and 16 additional amino acid residues, HisTag. Source of immunogen E.coli
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Constituents: 0.134% PBS, 0.58% Sodium chloride -
Concentration information loading...
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PurityImmunogen affinity purified
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Purification notesImmunoaffinity chromatography on a column with immobilized recombinant human Phospholipase A2 IID.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab47118 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | 1/50. PubMed: 22240557 | |
Functional Studies | Use a concentration of 2 µg/ml. PubMed: 22240557 | |
ELISA | Use at an assay dependent dilution. | |
WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa. |
Target
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FunctionPA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. L-alpha-1-palmitoyl-2-linoleoyl phosphatidylethanolamine is more efficiently hydrolyzed than the other phospholipids examined.
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Tissue specificityBroadly expressed.
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Sequence similaritiesBelongs to the phospholipase A2 family.
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Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 26279 Human
- Omim: 605630 Human
- SwissProt: Q9UNK4 Human
- Unigene: 189507 Human
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Alternative names
- 2IID antibody
- EC 3.1.1.4 antibody
- GIID sPLA2 antibody
see all
Datasheets and documents
References
This product has been referenced in:
- Mosher AA et al. Regulation of sPLA2-IID in Human Decidua: Insights Into the Complexity of the Prostaglandin Pathway in Labor. Reprod Sci N/A:N/A (2014). Human . Read more (PubMed: 24440994) »
- Li K et al. Secretory Phospholipase A2 Group IID Is Involved in Progesterone-Induced Acrosomal Exocytosis of Human Spermatozoa. J Androl 33:975-83 (2012). WB, ICC/IF, Functional Studies ; Human . Read more (PubMed: 22240557) »