Product nameAnti-Phospholipase A2 X antibody
See all Phospholipase A2 X primary antibodies
DescriptionRabbit polyclonal to Phospholipase A2 X
SpecificityThis antibody is specific for Phospholipase A2 X.
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Human
Recombinant human Phospholipase A2 X. A 15.5 kDa protein containing 123 amino acid residues of the human Phospholipase A2 X and 16 additional amino acid residues, HisTag. Source of immunogen, E.coli
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Constituents: 0.134% PBS, 0.58% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesImmunoaffinity chromatography on a column with immobilized recombinant human Phospholipase A2 X.
Our Abpromise guarantee covers the use of ab47111 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa.|
FunctionPA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Has a powerful potency for releasing arachidonic acid from cell membrane phospholipids. Prefers phosphatidylethanolamine and phosphatidylcholine liposomes to those of phosphatidylserine.
Tissue specificityFound in spleen, thymus, peripheral blood leukocytes, pancreas, lung, and colon.
Sequence similaritiesBelongs to the phospholipase A2 family.
- Information by UniProt
- Group 10 secretory phospholipase A2 antibody
- Group X secretory phospholipase A2 antibody
- GX sPLA2 antibody
All lanes : Anti-Phospholipase A2 X antibody (ab47111) at 1 µg/ml
Lane 1 : Whole cell extracts from RAW264.7 control cells
Lane 2 : Whole cell extracts from RAW264.7 cells overexpressing Phospholipase A2 X
Lysates/proteins at 20 µg per lane.
All lanes : Horseradish peroxidase-conjugated secondary antibody
Predicted band size: 17 kDa
Observed band size: 17 kDa
This product has been referenced in:
- Curfs DM et al. Macrophage Secretory Phospholipase A2 Group X Enhances Anti-inflammatory Responses, Promotes Lipid Accumulation, and Contributes to Aberrant Lung Pathology. J Biol Chem 283:21640-8 (2008). WB ; Human . Read more (PubMed: 18511424) »