Product nameAnti-Phospholipase A2 X antibody [EPR11202]
See all Phospholipase A2 X primary antibodies
DescriptionRabbit monoclonal [EPR11202] to Phospholipase A2 X
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC/IF or IP
Species reactivityReacts with: Mouse, Rat, Human
A recombinant protein fragment corresponding to a region of Human Phospholipase A2 X (UniProt ID: O15496).
- TT, A549, MCF7 and Caco 2 cell lysates; Human pancreas and tonsil tissues; permeabilized A549 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab166634 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 17 kDa.|
|IHC-P||1/100 - 1/250.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionPA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Has a powerful potency for releasing arachidonic acid from cell membrane phospholipids. Prefers phosphatidylethanolamine and phosphatidylcholine liposomes to those of phosphatidylserine.
Tissue specificityFound in spleen, thymus, peripheral blood leukocytes, pancreas, lung, and colon.
Sequence similaritiesBelongs to the phospholipase A2 family.
- Information by UniProt
- Group 10 secretory phospholipase A2 antibody
- Group X secretory phospholipase A2 antibody
- GX sPLA2 antibody
All lanes : Anti-Phospholipase A2 X antibody [EPR11202] (ab166634) at 1/1000 dilution
Lane 1 : TT cell lysate
Lane 2 : A549 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : Caco 2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 17 kDa
Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling Phospholipase A2 X with ab166634 at a 1/100 dilution.
Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling Phospholipase A2 X with ab166634 at a 1/100 dilution.
Flow cytometric analysis of permeabilized A549 cells using ab166634 at a 1/100 dilution (red) or a rabbit IgG (negative) (green).
This product has been referenced in:
- Zhang D et al. The inhibitory effect of saPLI?, a snake sourced PLA2 inhibitor on carrageenan-induced inflammation in mice. Toxicon 151:89-95 (2018). Read more (PubMed: 30003915) »
- Nolin JD et al. Secreted PLA2 group X orchestrates innate and adaptive immune responses to inhaled allergen. JCI Insight 2:N/A (2017). WB ; Mouse . Read more (PubMed: 29093264) »