Key features and details
- Rabbit polyclonal to Phospholipase A2 XII/PLA2G12A
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Phospholipase A2 XII/PLA2G12A antibody
See all Phospholipase A2 XII/PLA2G12A primary antibodies
DescriptionRabbit polyclonal to Phospholipase A2 XII/PLA2G12A
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow, Rhesus monkey
Recombinant fragment within Human Phospholipase A2 XII/PLA2G12A (internal sequence). The exact sequence is proprietary.
Database link: Q9BZM1
- IHC-P: Human colon carcinoma tissue. WB: HeLa whole cell lysate.
This product was previously labelled as Phospholipase A2 XII
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227477 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 21 kDa.|
FunctionPA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Does not exhibit detectable activity toward sn-2-arachidonoyl- or linoleoyl-phosphatidylcholine or -phosphatidylethanolamine.
Tissue specificityAbundantly expressed in heart, skeletal muscle, kidney, liver and pancreas.
Sequence similaritiesBelongs to the phospholipase A2 family.
Cellular localizationSecreted. Cytoplasm.
- Information by UniProt
- EC 18.104.22.168 antibody
- Group XII secreted phospholipase A2 antibody
- Group XIIA secreted phospholipase A2 antibody
Paraffin-embedded human colon carcinoma tissue stained for Phospholipase A2 XII/PLA2G12A using ab227477 at 1/500 dilution in immunohistochemical analysis.
Anti-Phospholipase A2 XII/PLA2G12A antibody (ab227477) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 21 kDa
12% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227477 has not yet been referenced specifically in any publications.