Key features and details
- Mouse monoclonal [bc-01] to Phospholipase C
- Suitable for: WB, ELISA
- Reacts with: Species independent
- Isotype: IgM
Product nameAnti-Phospholipase C antibody [bc-01]
DescriptionMouse monoclonal [bc-01] to Phospholipase C
Reacts with phosphatidylcholine phospholipase C (PC-PLC) of Bacillus cereus.
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Species independent
Tissue, cells or virus corresponding to Phospholipase C. Culture supernatant of B. cereus.
Database link: P09598
- WB: Culture medium of B. cereus. PC - PLC purified from B. cereus. ELISA: Crude extract of B. cereus.
This product was previously labelled as Phosphilipase C
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab243181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000.|
|ELISA||Use at an assay dependent concentration.|
All lanes : Anti-Phospholipase C antibody [bc-01] (ab243181) at 1/1000 dilution
Lane 1 : Culture medium of B. cereus
Lane 2 : PC-PLC purified from B. cereus
Titration ab243181 by indirect ELISA using crude extract of B. cereus.
An ELISA plate was coated with crude extract of B. cereus (100 µL, 1 µg/mL). After blocking with 5% skimmed milk, 100 µL of antibody at the indicated dilutions was added to each well. HRP-conjugated goat anti-mouse IgM (100 µL, X2000 dilution) was added.
Titration of PLC in the extract of B. cereus cells by indirect ELISA using ab243181.
An ELISA plate was coated with indicated amounts of the extract of B. cereus cells per well.
ab243181 was used at a 1/500 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab243181 has been referenced in 2 publications.
- Duliban M et al. Interstitial Leydig Cell Tumorigenesis-Leptin and Adiponectin Signaling in Relation to Aromatase Expression in the Human Testis. Int J Mol Sci 21:N/A (2020). PubMed: 32455738
- Shen X et al. Metformin Reduces Lipotoxicity-Induced Meta-Inflammation in ß-Cells through the Activation of GPR40-PLC-IP3 Pathway. J Diabetes Res 2019:7602427 (2019). PubMed: 31950065