Anti-Phospholipase C antibody [bc-01] (ab243181)

Overview

  • Product name

    Anti-Phospholipase C antibody [bc-01]
  • Description

    Mouse monoclonal [bc-01] to Phospholipase C
  • Host species

    Mouse
  • Specificity

    Reacts with phosphatidylcholine phospholipase C (PC-PLC) of Bacillus cereus.

  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Tissue, cells or virus corresponding to Phospholipase C. Culture supernatant of B. cereus.
    Database link: P09598

  • Positive control

    • WB: Culture medium of B. cereus. PC - PLC purified from B. cereus. ELISA: Crude extract of B. cereus.
  • General notes

     This product was previously labelled as Phosphilipase C

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    bc-01
  • Isotype

    IgM

Applications

Our Abpromise guarantee covers the use of ab243181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000.
ELISA Use at an assay dependent concentration.

Images

  • All lanes : Anti-Phospholipase C antibody [bc-01] (ab243181) at 1/1000 dilution

    Lane 1 : Culture medium of B. cereus
    Lane 2 : PC-PLC purified from B. cereus
  • Titration ab243181 by indirect ELISA using crude extract of B. cereus.

    An ELISA plate was coated with crude extract of B. cereus (100 µL, 1 µg/mL). After blocking with 5% skimmed milk, 100 µL of antibody at the indicated dilutions was added to each well. HRP-conjugated goat anti-mouse IgM (100 µL, X2000 dilution) was added.

  • Titration of PLC in the extract of B. cereus cells by indirect ELISA using ab243181.

    An ELISA plate was coated with indicated amounts of the extract of B. cereus cells per well.

    ab243181 was used at a 1/500 dilution.

References

ab243181 has not yet been referenced specifically in any publications.

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