Recombinant
RabMAb

Recombinant Anti-Phospholipase C beta 1/PLCB1 antibody [EPR18895] (ab182368)

Overview

  • Product name

    Anti-Phospholipase C beta 1/PLCB1 antibody [EPR18895]
    See all Phospholipase C beta 1/PLCB1 primary antibodies
  • Description

    Rabbit monoclonal [EPR18895] to Phospholipase C beta 1/PLCB1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Mouse Phospholipase C beta 1/PLCB1 aa 950 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9Z1B3

  • Positive control

    • WB: Mouse brain lysate; Human fetal brain lysate; Rat brain lysate; C6 whole cell lysate. IHC-P: Human, mouse and rat cerebrum tissues. IP: Rat brain whole cell lysate.
  • General notes

     

     

     This product was previously labelled as Phospholipase C beta 1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR18895
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab182368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 138 kDa).
IP 1/20.

Target

  • Function

    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.
  • Involvement in disease

    Epileptic encephalopathy, early infantile, 12
  • Sequence similarities

    Contains 1 C2 domain.
    Contains 1 PI-PLC X-box domain.
    Contains 1 PI-PLC Y-box domain.
  • Cellular localization

    Nucleus membrane. Cytoplasm. Colocalizes with the adrenergic receptors, ADREN1A and ADREN1B, at the nuclear membrane of cardiac myocytes.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1 phosphatidylinositol 4,5 bisphosphate phosphodiesterase beta 1 antibody
    • 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate antibody
    • 1-phosphatidylinositol 4 antibody
    • 1-phosphatidylinositol-4 antibody
    • 5-bisphosphate phosphodiesterase beta-1 antibody
    • EIEE12 antibody
    • Inositoltrisphosphohydrolase antibody
    • Monophosphatidylinositol phosphodiesterase antibody
    • Phosphb antibody
    • Phosphoinositidase C antibody
    • Phosphoinositide phospholipase C antibody
    • Phosphoinositide phospholipase C-beta 1 antibody
    • Phosphoinositide phospholipase C-beta-1 antibody
    • Phospholipase C beta 1 (phosphoinositide-specific) antibody
    • Phospholipase C I antibody
    • Phospholipase C-beta-1 antibody
    • Phospholipase C-I antibody
    • PI PLC antibody
    • PLC 1 antibody
    • PLC beta 1 antibody
    • PLC I antibody
    • PLC-154 antibody
    • PLC-beta-1 antibody
    • PLC-I antibody
    • PLC154 antibody
    • Plcb antibody
    • Plcb1 antibody
    • Plcb1 protein antibody
    • PLCB1_HUMAN antibody
    • PLCbeta1 antibody
    • Triphosphoinositide phosphodiesterase antibody
    see all

Images

  • All lanes : Anti-Phospholipase C beta 1/PLCB1 antibody [EPR18895] (ab182368) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Mouse spleen lysate
    Lane 5 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 7 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 138 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time 3 minutes; GAPDH control lanes: 15 seconds.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 8429045)
    PLCB1 is selectively expressed in brain which has been described in the literature
    (PMID: 2468162)

  • Anti-Phospholipase C beta 1/PLCB1 antibody [EPR18895] (ab182368) at 1/1000 dilution + Human fetal brain lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 138 kDa
    Observed band size: 150 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 8429045)

  • All lanes : Anti-Phospholipase C beta 1/PLCB1 antibody [EPR18895] (ab182368) at 1/1000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Rat heart lysate
    Lane 3 : Rat kidney lysate
    Lane 4 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 138 kDa
    Observed band size: 150 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID:) 8429045
    PLCB1 is selectively expressed in brain which has been described in the literature
    (PMID: 2468162)

  • Anti-Phospholipase C beta 1/PLCB1 antibody [EPR18895] (ab182368) at 1/1000 dilution + C6 (Rat glial tumor cells) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 138 kDa
    Observed band size: 150 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The double bands represent two isoforms of the target which have been described in the literature (PMID: 7510682).

  • Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Phospholipase C beta 1/PLCB1 with ab182368 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Mainly cytoplasm staining on normal Human cerebrum is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Gastric adenocarcinoma tissue labeling Phospholipase C beta 1/PLCB1 with ab182368 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on gastric adenocarcinoma. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Phospholipase C beta 1/PLCB1 with ab182368 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on mouse cerebrum is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling Phospholipase C beta 1/PLCB1 with ab182368 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on mouse stomach. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Phospholipase C beta 1/PLCB1 with ab182368 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on rat cerebrum is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Phospholipase C beta 1/PLCB1 with ab182368 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on rat stomach. Counter stained with Hematoxylin.

     

     

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Phospholipase C beta 1/PLCB1 was immunoprecipitated from 1mg of Rat brain whole cell lysate with ab182368 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab182368 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
    Lane 1: Rat brain whole cell lysate 10ug (Input).
    Lane 2: ab182368 IP in Rat brain whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab182368 in Rat brain whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 minutes.

References

ab182368 has not yet been referenced specifically in any publications.

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